Rabbit anti-Human Myelin Protein Zero Polyclonal Antibody | anti-MPZ antibody

Myelin Protein Zero, CT (Myelin Protein P0, MPZ, P0, CHM, CMT1, CMT1B, CMT2I, CMT2J, CMT4E, CMTDI3, DSS, HMSNIB, Myelin Peripheral Protein, MPP) (FITC)

Cat. Num. AAA6488888

• Gene Names: MPZ; P0; CHM; DSS; MPP; CMT1; CMT1B; CMT2I; CMT2J; CMT4E; CMTDI3; CMTDID; HMSNIB
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Myelin Protein Zero; Polyclonal Antibody; CT (Myelin Protein P0; MPZ; P0; CHM; CMT1; CMT1B; CMT2I; CMT2J; CMT4E; CMTDI3; DSS; HMSNIB; Myelin Peripheral Protein; MPP) (FITC); Myelin Protein Zero (Charcot-Marie-Tooth Neuropathy 1B); anti-MPZ antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MPZ.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MPZ antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MPZ (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MPZ Antibody (C-term) in CEM cell line lysates (35ug/lane). MPZ (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(MPZ Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(MPZ Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MPZ Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of MPZ (arrow) using rabbit polyclonal MPZ Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MPZ gene.)

Product Categories/Family for anti-MPZ antibody

Antibodies; Neuroscience

References

1. Tachi, N., et.al., Brain Dev. 6(6), 560-565 (1984). 2. Hayasaka, K., et.al., Biochem. Biophys. Res. Commun. 180(2), 515-518 (1991). 3. Hayasaka, K., et.al., Genomics 17(3), 755-758 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 4505243
• NCBI GeneID: 4359
• NCBI Accession #: NP_000521.1
• NCBI GenBank Nucleotide #: NM_000530.5
• UniProt Accession #: P25189; Q16072; Q5VTH4; Q92677; Q9BR67
• Molecular Weight: 34,387 Da
• NCBI Official Full Name: myelin protein P0
• NCBI Official Synonym Full Names: myelin protein zero
• NCBI Official Symbol: MPZ
• NCBI Official Synonym Symbols: P0; CHM; DSS; MPP; CMT1; CMT1B; CMT2I; CMT2J; CMT4E; CMTDI3; CMTDID; HMSNIB
• NCBI Protein Information: myelin protein P0
• UniProt Protein Name: Myelin protein P0
• Protein Family: Myelin protein
• UniProt Gene Name: MPZ
• UniProt Synonym Gene Names: MPP
• UniProt Entry Name: MYP0_HUMAN

NCBI Description

This gene is specifically expressed in Schwann cells of the peripheral nervous system and encodes a type I transmembrane glycoprotein that is a major structural protein of the peripheral myelin sheath. The encoded protein contains a large hydrophobic extracellular domain and a smaller basic intracellular domain, which are essential for the formation and stabilization of the multilamellar structure of the compact myelin. Mutations in this gene are associated with autosomal dominant form of Charcot-Marie-Tooth disease type 1 (CMT1B) and other polyneuropathies, such as Dejerine-Sottas syndrome (DSS) and congenital hypomyelinating neuropathy (CHN). A recent study showed that two isoforms are produced from the same mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Oct 2015]

Uniprot Description

myelin P0: a structural protein in peripheral nervous system Schwann cells. Forms an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae. Defects cause of Charcot-Marie-Tooth disease, Dejerine-Sottas syndrome, congenital hypomyelination neuropathy, and Roussy-Levy syndrome.

Protein type: Adaptor/scaffold; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: integral to plasma membrane; plasma membrane; myelin sheath

Molecular Function: structural molecule activity

Biological Process: synaptic transmission; intercellular junction maintenance

Disease: Neuropathy, Congenital Hypomyelinating Or Amyelinating, Autosomal Recessive; Charcot-marie-tooth Disease, Demyelinating, Type 1b; Hypertrophic Neuropathy Of Dejerine-sottas; Charcot-marie-tooth Disease, Dominant Intermediate D; Charcot-marie-tooth Disease, Axonal, Type 2j; Charcot-marie-tooth Disease, Axonal, Type 2i; Roussy-levy Hereditary Areflexic Dystasia