Rabbit anti-Human, Mouse MSRA Polyclonal Antibody | anti-MSRA antibody

MSRA, NT (Methionine Sulfoxide Reductase A, Peptide Methionine Sulfoxide Reductase, Peptide-methionine (S)-S-oxide Reductase, Peptide Met(O) Reductase, Protein-methionine-S-oxide Reductase, PMSR) (MaxLight 405)

Cat. Num. AAA6488626

• Gene Names: MSRA; PMSR
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: MSRA; Polyclonal Antibody; NT (Methionine Sulfoxide Reductase A; Peptide Methionine Sulfoxide Reductase; Peptide-methionine (S)-S-oxide Reductase; Peptide Met(O) Reductase; Protein-methionine-S-oxide Reductase; PMSR) (MaxLight 405); EC=1.8.4.11; anti-MSRA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MSRA. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-MSRA antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human MSRA (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MSRA antibody (N-term) in mouse kidney tissue lysates (35ug/lane). MSRA (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MSRA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MSRA Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-MSRA antibody

Antibodies; Enzymes; Reductase

References

1. Pascual, I., Larrayoz, I.M. Genomics 93(1), 62-71 (2009). 2. Schallreuter, K.U., Rubsam, K. J. Invest. Dermatol. 128(4), 808-815 (2008). 3. Picot, C.R., Perichon, M. FEBS Lett. 558(1-3), 74-78 (2004). 4. Vougier, S., Mary, J. Biochem. J. 373(PT 2), 531-537 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 6912516
• NCBI GeneID: 4482
• NCBI Accession #: NP_036463
• NCBI GenBank Nucleotide #: NM_012331.4
• UniProt Accession #: Q9UJ68; Q52TC4; Q549N4; Q66MI7; E9PAS8
• Molecular Weight: 26.2kDa (237aa), confirmed by MALDI-TOF
• NCBI Official Full Name: mitochondrial peptide methionine sulfoxide reductase isoform a
• NCBI Official Synonym Full Names: methionine sulfoxide reductase A
• NCBI Official Symbol: MSRA
• NCBI Official Synonym Symbols: PMSR
• NCBI Protein Information: mitochondrial peptide methionine sulfoxide reductase
• UniProt Protein Name: Mitochondrial peptide methionine sulfoxide reductase
• Protein Family: Mitochondrial peptide methionine sulfoxide reductase
• UniProt Gene Name: MSRA
• UniProt Synonym Gene Names: Peptide Met(O) reductase; PMSR
• UniProt Entry Name: MSRA_HUMAN

NCBI Description

This gene encodes a ubiquitous and highly conserved protein that carries out the enzymatic reduction of methionine sulfoxide to methionine. Human and animal studies have shown the highest levels of expression in kidney and nervous tissue. The protein functions in the repair of oxidatively damaged proteins to restore biological activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

MSRA: Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. Belongs to the MsrA Met sulfoxide reductase family. 5 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 1.8.4.11; Oxidoreductase

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: nucleoplasm; mitochondrion; membrane; cytoplasm; actin cytoskeleton

Molecular Function: peptide-methionine-(S)-S-oxide reductase activity

Biological Process: protein repair; protein modification process; methionine metabolic process; response to oxidative stress