Rabbit anti-Human MLX Polyclonal Antibody | anti-MLX antibody

MLX, ID (Max-like Protein X, Max-like bHLHZip Protein, BigMax Protein, Transcription Factor-like Protein 4, Class D Basic Helix-loop-helix Protein 13, bHLHd13, TCFL4) (Azide free) (HRP)

Cat. Num. AAA6488427

• Gene Names: MLX; MAD7; MXD7; TCFL4; bHLHd13
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: MLX; Polyclonal Antibody; ID (Max-like Protein X; Max-like bHLHZip Protein; BigMax Protein; Transcription Factor-like Protein 4; Class D Basic Helix-loop-helix Protein 13; bHLHd13; TCFL4) (Azide free) (HRP); anti-MLX antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MLX.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MLX antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human MLX (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MLX Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). MLX (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with MLX Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H292 cells using MLX Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of MLX (arrow) using rabbit polyclonal MLX Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLX gene.)

Product Categories/Family for anti-MLX antibody

Antibodies; Transcription Factors

References

1. Meroni, G., et.al., Oncogene 19(29), 3266-3277 (2000). 2. Billin, A.N., et.al., J. Biol. Chem. 274(51), 36344-36350 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 24586667
• NCBI GeneID: 6945
• NCBI Accession #: NP_733752.1
• NCBI GenBank Nucleotide #: NM_170607.2
• UniProt Accession #: Q9UH92; Q53XM6; Q96FL2; Q9H2V0; Q9H2V1; Q9H2V2; Q9NXN3; A8K2J3; B2RAV8; B2RD73
• Molecular Weight: 33,300 Da
• NCBI Official Full Name: max-like protein X isoform gamma
• NCBI Official Synonym Full Names: MLX, MAX dimerization protein
• NCBI Official Symbol: MLX
• NCBI Official Synonym Symbols: MAD7; MXD7; TCFL4; bHLHd13
• NCBI Protein Information: max-like protein X; BigMax protein; MAX-like bHLHZIP protein; transcription factor-like protein 4; class D basic helix-loop-helix protein 13
• UniProt Protein Name: Max-like protein X
• Protein Family: Max-like protein
• UniProt Gene Name: MLX
• UniProt Synonym Gene Names: BHLHD13; TCFL4; bHLHd13
• UniProt Entry Name: MLX_HUMAN

NCBI Description

The product of this gene belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This gene product may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MLX: Transcription regulator. Forms a sequence-specific DNA- binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; transcription factor activity; transcription factor binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; energy reserve metabolic process; positive regulation of cellular metabolic process; nucleocytoplasmic transport; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent