Rabbit anti-Human, Mouse MBD3 Polyclonal Antibody | anti-MBD3 antibody

MBD3, CT (Methyl-CpG-binding Domain 3, Methyl-CpG-binding Protein MBD3) (PE)

Cat. Num. AAA6487270

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: MBD3; Polyclonal Antibody; CT (Methyl-CpG-binding Domain 3; Methyl-CpG-binding Protein MBD3) (PE); anti-MBD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MBD3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MBD3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding E279 of human MBD3 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MBD3 (arrow) using rabbit polyclonal MBD3 Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MBD3 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-MBD3 antibody

Antibodies; Binding Proteins

References

1. Bhattacharya SK, Ramchandani S, Cervoni N, Szyf. M. Nature, 397(6720):579-583 1999. 2. Hendrich B and Bird A. Mol Cell Biol, 18: 6538-6547(1998). 3. Petronzelli F, Riccio A, Markham GD, Seeholzer SH, Stoerker J, Genuardi M, Yeung AT, Matsumoto Y, Bellacosa A. J Biol Chem 275(42): 32422-32429 (2000). 4. Bader S, Walker M, Harrison D. Br J Cancer 83(12): 1646-1649 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 4505119
• NCBI GeneID: 53615
• NCBI Accession #: NP_003917.1
• NCBI GenBank Nucleotide #: NM_003926.5
• UniProt Accession #: O95983; Q6PIL9; Q6PJZ9; Q86XF4; A8K4B7; D6W5Z2
• Molecular Weight: 32,844 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 3
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 3
• NCBI Official Symbol: MBD3
• NCBI Protein Information: methyl-CpG-binding domain protein 3
• UniProt Protein Name: Methyl-CpG-binding domain protein 3
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD3
• UniProt Entry Name: MBD3_HUMAN

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. [provided by RefSeq, Jul 2008]

Uniprot Description

MBD3: methyl-CpG binding (MBD) proteins appear to bind differentially methylated domains (DMDs) of DNA, to recruit repression complexes, and to silence the locus. The methyl CpG binding domain of human MBD3 may not directly bind mCpG but does interact with the NuRD/Mi2 components HDAC1 and MTA2. Plays a role in histone deacetylation and nucleosome remodeling. Increased levels have been reported in lung cancer and glioblastomas and may be a therapeutic target.

Protein type: Gene silencing; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; heterochromatin; protein complex; nuclear chromatin; cytoplasm; NuRD complex

Molecular Function: protein binding; nucleosomal DNA binding; DNA binding; methyl-CpG binding

Biological Process: tissue development; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; methylation-dependent chromatin silencing; in utero embryonic development; ATP-dependent chromatin remodeling; negative regulation of transcription from RNA polymerase II promoter; histone acetylation