Rabbit anti-Human MAP Kinase Interacting Serine/threonine Kinase 1 Polyclonal Antibody | anti-MNK1 antibody

MAP Kinase Interacting Serine/threonine Kinase 1, NT (MAP Kinase Signal-integrating Kinase 1, MAPK Signal-integrating Kinase 1, MKNK1, MNK1) (AP)

Cat. Num. AAA6486616

• Gene Names: MKNK1; MNK1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: MAP Kinase Interacting Serine/threonine Kinase 1; Polyclonal Antibody; NT (MAP Kinase Signal-integrating Kinase 1; MAPK Signal-integrating Kinase 1; MKNK1; MNK1) (AP); MAP Kinase-interacting Serine/threonine-protein Kinase 1; EC=2.7.11.1; anti-MNK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MKNK1.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-MNK1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids1-30 from the N-terminal region of human MKNK1 (NP_003675; Q9BUB5).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of A2058 tissue lysate using M2353-70N)

Product Categories/Family for anti-MNK1 antibody

Antibodies; Protein Kinases

References

1. Orton, K.C., et al., J. Biol. Chem. 279(37):38649-38657 (2004). 2. Cuesta, R., et al., J. Virol. 78(14):7707-7716 (2004). 3. Knauf, U., et al., Mol. Cell. Biol. 21(16):5500-5511 (2001). 4. Cuesta, R., et al., Genes Dev. 14(12):1460-1470 (2000). 5. Fukunaga, R., et al., EMBO J. 16(8):1921-1933 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 1358101397
• NCBI GeneID: 8569
• NCBI Accession #: NP_003675
• NCBI GenBank Nucleotide #: NM_003684
• UniProt Accession #: Q9BUB5
• Molecular Weight: 51kDa
• NCBI Official Full Name: MAP kinase-interacting serine/threonine-protein kinase 1 isoform 1
• NCBI Official Synonym Full Names: MAP kinase interacting serine/threonine kinase 1
• NCBI Official Symbol: MKNK1
• NCBI Official Synonym Symbols: MNK1
• NCBI Protein Information: MAP kinase-interacting serine/threonine-protein kinase 1
• UniProt Protein Name: MAP kinase-interacting serine/threonine-protein kinase 1
• UniProt Gene Name: MKNK1
• UniProt Synonym Gene Names: MNK1; MAPK signal-integrating kinase 1; Mnk1
• UniProt Entry Name: MKNK1_HUMAN

NCBI Description

This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Mnk1: a CAM kinase of the MAPKKAPK family that can regulate translation. Following activation by MAP kinases, Mnk1 phosphorylates eIF4E at Ser209, increasing its affinity for the 7-methylguanosine-containing mRNA cap. Two MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for its kinase activity. Three alternatively spliced human isoforms have been described.

Protein type: Protein kinase, CAMK; EC 2.7.11.1; Translation; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; MAPKAPK family; MNK subfamily

Chromosomal Location of Human Ortholog: 1p33

Cellular Component: nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: peptidyl-serine phosphorylation; fibroblast growth factor receptor signaling pathway; protein amino acid phosphorylation; response to salt stress; regulation of translational initiation