Rabbit anti-Human Laminin Polyclonal Antibody | anti-LAMA1 antibody

Laminin (APC)

Cat. Num. AAA6484680

• Gene Names: LAMA1; LAMA; S-LAM-alpha
• Reactivity: Human
• Applications: ELISA, Immunoblot, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Laminin; Polyclonal Antibody; Laminin (APC); anti-LAMA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes most mammalian laminins and has negligible crossreactivity with Type I, II, III, IV, V or VI collagens or fibronectin. Non-specific crossreaction of anti-laminin antibodies with other human serum proteins or non-laminin extracellular matrix proteins is negligible. Typically less than 1% crossreactivity against other extracellular matrix proteins was detected by ELISA against purified standards.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-LAMA1 antibody: ELISA (EIA), Immunoblot, Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: ELISA: 1:2000-1:4000; Immunoblot: 1:2500-1:5000; IHC: 1:25-1:100; Applications are based on unconjugated antibody.
• Immunogen: Full length human laminin. Immunization occurred after single-band purity was assessed by SDS-PAGE.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-LAMA1 antibody

Antibodies; Lamin; Laminin

References

1. Journal of Vascular Research (1999). 36: 47-58.

NCBI and Uniprot Product Information

• NCBI GI #: 38788416
• NCBI GeneID: 284217
• NCBI Accession #: NP_005550.2
• NCBI GenBank Nucleotide #: NM_005559.2
• UniProt Accession #: P25391
• Molecular Weight: 337,084 Da
• NCBI Official Full Name: laminin subunit alpha-1
• NCBI Official Synonym Full Names: laminin, alpha 1
• NCBI Official Symbol: LAMA1
• NCBI Official Synonym Symbols: LAMA; S-LAM-alpha
• NCBI Protein Information: laminin subunit alpha-1; S-LAM alpha; laminin A chain; S-laminin subunit alpha; laminin-3 subunit alpha
• UniProt Protein Name: Laminin subunit alpha-1
• Protein Family: Laminin
• UniProt Gene Name: LAMA1
• UniProt Synonym Gene Names: LAMA; S-LAM alpha
• UniProt Entry Name: LAMA1_HUMAN

NCBI Description

This gene encodes one of the alpha 1 subunits of laminin. The laminins are a family of extracellular matrix glycoproteins that have a heterotrimeric structure consisting of an alpha, beta and gamma chain. These proteins make up a major component of the basement membrane and have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Mutations in this gene may be associated with Poretti-Boltshauser syndrome. [provided by RefSeq, Sep 2014]

Uniprot Description

LAMA1: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Protein type: Secreted; Extracellular matrix; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 18p11.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; laminin-3 complex; laminin-1 complex; extracellular region; basement membrane; intercellular junction

Molecular Function: protein binding; extracellular matrix structural constituent; glycosphingolipid binding; receptor binding

Biological Process: regulation of cell adhesion; axon guidance; extracellular matrix organization and biogenesis; cell surface receptor linked signal transduction; regulation of embryonic development; morphogenesis of an epithelial sheet; establishment of epithelial cell polarity; cell adhesion; regulation of cell migration

Disease: Poretti-boltshauser Syndrome