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Western Blot (WB) (WB: HeLa nuclear extract was probed with anti-Kip1 (p27) (0.5ug/ml). Arrow indicates Kip1 (p27).)

Rabbit Kip1 Polyclonal Antibody | anti-Kip1 antibody

Kip1 p57 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor) (APC)

Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human, Rabbit, Rat
Applications
Immunohistochemistry, Western Blot, Immunoprecipitation
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Kip1; Polyclonal Antibody; Kip1 p57 (cdk Inhibitor; Cyclin Dependent Kinase Inhibitor) (APC); anti-Kip1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rabbit, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Kip1 p57. Species crossreactivity: Rabbit and rat.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-Kip1 antibody
Immunohistochemistry (IHC) Frozen, Western Blot (WB), Immunoprecipitation (IP)
Application Notes
IHC-F: 5ug/ml showed positive immunostaining for Kip1 in formalin-fixed, paraffin embedded rat brain tissue.
WB: 1ug/ml detected Kip1 (~27kD) in a HeLa nuclear extract and a rabbit brain nuclear extract.
IP: 4ug immunoprecipitated Kip1 from a HeLa nuclear extract and a rabbit brain nuclear extract.
Applications are based on unconjugated antibody.
Immunogen
An 18 amino acid synthetic peptide ([C]GSVEQTPKKPGLRRRQT) linked to KLH was used to immunize rabbits. The human Kip1 sequence used (amino acids 182-198) shares 13/17 amino acids with murine Kip1. No other known proteins share more than 8 amino acids with the sequence.
Conjugate
APC
Positive Control
HeLa nuclear extract cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(WB: HeLa nuclear extract was probed with anti-Kip1 (p27) (0.5ug/ml). Arrow indicates Kip1 (p27).)

Western Blot (WB) (WB: HeLa nuclear extract was probed with anti-Kip1 (p27) (0.5ug/ml). Arrow indicates Kip1 (p27).)
Product Categories/Family for anti-Kip1 antibody
References
Polyak, K., et al., Genes and Development 8: 9-22, 1994. Peters, G., Nature 371: 204-205, 1994.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,073 Da
NCBI Official Full Name
cyclin-dependent kinase inhibitor 1B
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 1B (p27, Kip1)
NCBI Official Symbol
CDKN1B
NCBI Official Synonym Symbols
KIP1; MEN4; CDKN4; MEN1B; P27KIP1
NCBI Protein Information
cyclin-dependent kinase inhibitor 1B
UniProt Protein Name
Cyclin-dependent kinase inhibitor 1B
Protein Family
UniProt Gene Name
CDKN1B
UniProt Synonym Gene Names
KIP1
UniProt Entry Name
CDN1B_HUMAN

NCBI Description

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq, Jul 2008]

Uniprot Description

p27Kip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. May mediate TGF beta-induced g1 arrest. Its degradation is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Protein type: Oncoprotein; Cell cycle regulation; Inhibitor; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 12p13.1-p12

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus; endosome

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; chaperone binding; protein complex binding; caspase activator activity; Hsp70 protein binding; protein phosphatase binding; transforming growth factor beta receptor, cytoplasmic mediator activity

Biological Process: response to peptide hormone stimulus; negative regulation of kinase activity; nerve growth factor receptor signaling pathway; positive regulation of microtubule polymerization; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to estradiol stimulus; negative regulation of cell proliferation; sensory perception of sound; positive regulation of cell proliferation; response to glucose stimulus; negative regulation of mitotic cell cycle; cell cycle arrest; inner ear development; potassium ion transport; response to drug; caspase activation; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; negative regulation of cyclin-dependent protein kinase activity; negative regulation of cell motility; response to amino acid stimulus; response to cadmium ion; autophagic cell death; positive regulation of protein catabolic process; response to hypoxia; negative regulation of phosphorylation; innate immune response; negative regulation of cell growth; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; G1/S transition of mitotic cell cycle

Disease: Multiple Endocrine Neoplasia, Type Iv

Research Articles on Kip1

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Product Notes

The Kip1 cdkn1b (Catalog #AAA6484383) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Kip1 p57 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor) (APC) reacts with Human, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Kip1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Frozen, Western Blot (WB), Immunoprecipitation (IP). IHC-F: 5ug/ml showed positive immunostaining for Kip1 in formalin-fixed, paraffin embedded rat brain tissue. WB: 1ug/ml detected Kip1 (~27kD) in a HeLa nuclear extract and a rabbit brain nuclear extract. IP: 4ug immunoprecipitated Kip1 from a HeLa nuclear extract and a rabbit brain nuclear extract. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Kip1 cdkn1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kip1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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