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Western Blot (WB) (Western blot analysis of AVIL Antibody (N-term) in K562 cell line lysates (35ug/lane). AVIL (arrow) was detected using the purified Pab.)

Rabbit anti-Human AVIL Polyclonal Antibody | anti-AVIL antibody

AVIL, NT (AVIL, Advillin, p92)

Gene Names
AVIL; p92; DOC6; ADVIL
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
AVIL; Polyclonal Antibody; NT (AVIL; Advillin; p92); Anti -AVIL; anti-AVIL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-AVIL antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
AVIL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183~212 amino acids from the N-terminal region of human AVIL.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of AVIL Antibody (N-term) in K562 cell line lysates (35ug/lane). AVIL (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of AVIL Antibody (N-term) in K562 cell line lysates (35ug/lane). AVIL (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(AVIL Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AVIL Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (AVIL Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AVIL Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(AVIL Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (AVIL Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-AVIL antibody
AVIL is a member of the gelsolin/villin family of actin regulatory proteins. This protein has structural similarity to villin. It binds actin and may play a role in the development of neuronal cells that form ganglia.
Product Categories/Family for anti-AVIL antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
92,027 Da
NCBI Official Full Name
advillin
NCBI Official Synonym Full Names
advillin
NCBI Official Symbol
AVIL
NCBI Official Synonym Symbols
p92; DOC6; ADVIL
NCBI Protein Information
advillin
UniProt Protein Name
Advillin
Protein Family
UniProt Gene Name
AVIL
UniProt Entry Name
AVIL_HUMAN

NCBI Description

The protein encoded by this gene is a member of the gelsolin/villin family of actin regulatory proteins. This protein has structural similarity to villin. It binds actin and may play a role in the development of neuronal cells that form ganglia. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Ca2+-regulated actin-binding protein. May have a unique function in the morphogenesis of neuronal cells which form ganglia. Required for SREC1-mediated regulation of neurite-like outgrowth. Plays a role in regenerative sensory axon outgrowth and remodeling processes after peripheral injury in neonates. Involved in the formation of long fine actin-containing filopodia-like structures in fibroblast. Plays a role in ciliogenesis. Ref.7

Subunit structure: Associates (via C-terminus) with actin. Interacts with SCARF1

By similarity. Interacts with F-actin. Ref.8

Subcellular location: Cytoplasm › cytoskeleton. Cell projection

By similarity. Cell projection › axon

By similarity.

Tissue specificity: Most highly expressed in the small intestine and colonic lining. Weaker expression also detected in the thymus, prostate, testes and uterus. Ref.6

Sequence similarities: Belongs to the villin/gelsolin family.Contains 6 gelsolin-like repeats.Contains 1 HP (headpiece) domain.

Research Articles on AVIL

Similar Products

Product Notes

The AVIL avil (Catalog #AAA648218) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AVIL, NT (AVIL, Advillin, p92) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AVIL can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the AVIL avil for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AVIL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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