Rat anti-Mouse Endomucin Monoclonal Antibody | anti-EMCN antibody

Endomucin (Endomucin-1/2, Mucin-14, MUC-14, Emcn)

Cat. Num. AAA648172

• Gene Names: EMCN; EMCN2; MUC14
• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Endomucin; Monoclonal Antibody; Endomucin (Endomucin-1/2; Mucin-14; MUC-14; Emcn); Anti -Endomucin (Endomucin-1/2; anti-EMCN antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 13A45
• Specificity: Recognizes mouse Endomucin. Does not react with human.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

• Applicable Applications for anti-EMCN antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Flow Cytometry, Immunoprecipitation and Immunohistochemistry.; Dilution: Flow Cytometry: 1:50; Western Blot: 1:50; Immunohistochemistry: Frozen; Immunohistochemistry: Paraffin
• Immunogen: Mouse endomucin-IgG fusion protein
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-EMCN antibody

Endomucin is a type I membrane protein of 248aa (75kD) and shows no signicant homology to any known glycoprotein. As a typical mucin-like glycoprotein, endomucin has a high content of serine and threonine residues, suggesting strong O- glycosylation; the sensitivity to O-sialoglycoprotein endopeptidase indicates that endomucin is also a sialomucin. Endomucin is an endothelial-specific sialomucin. It is a constitutively expressed endothelial cell surface protein that is found on all venules but is absent from high endothelial venule cells (HEV) of peripheral and mesenteric lymph nodes as well as Peyer's patches, the specialized site for most efficient lymphocyte trafficking. This could indicate an anti-adhesive function of endomucin, as demonstrated for other sialomucins. Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) is another cell adhesion molecule that contains a mucin-like domain and is expressed on HEV in Peyer's patches, mesenteric lymph nodes and on venules in intestinal lamina propria. In the HEV of mesenteric lymph nodes, the mucin-like domain of a subpopulation of MAdCAM-1 molecules contains sulfated carbohydrate side chains that interact with L-selectin. The presence of three putative protein kinase C phosphorylation sites in the cytoplasmic tail of endomucin indicates that endomucin has the capacity to be a signaling molecule.

Product Categories/Family for anti-EMCN antibody

Antibodies; Abs to Mucin

NCBI and Uniprot Product Information

• NCBI GI #: 20070284
• NCBI GeneID: 51705
• NCBI Accession #: NP_057326.2
• NCBI GenBank Nucleotide #: NM_016242.3
• UniProt Accession #: Q9ULC0; Q8NEY5; Q8WWE7; Q9NRM8; A8K716; B4E347
• Molecular Weight: 27,452 Da
• NCBI Official Full Name: endomucin isoform 1
• NCBI Official Synonym Full Names: endomucin
• NCBI Official Symbol: EMCN
• NCBI Official Synonym Symbols: EMCN2; MUC14
• NCBI Protein Information: endomucin; MUC-14; mucin-14; endomucin-2; gastric cancer antigen Ga34
• UniProt Protein Name: Endomucin
• Protein Family: Endomucin
• UniProt Gene Name: EMCN
• UniProt Synonym Gene Names: EMCN2; MUC14; MUC-14
• UniProt Entry Name: MUCEN_HUMAN

NCBI Description

EMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (Kinoshita et al., 2001 [PubMed 11418125]).[supplied by OMIM, Mar 2008]

Uniprot Description

Function: Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Membrane; Single-pass type I membrane protein. Note: Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks.Isoform 2: Secreted

Potential.

Tissue specificity: Expressed in heart, kidney and lung. Ref.1 Ref.2

Post-translational modification: Highly O-glycosylated. Sialic acid-rich glycoprotein. Ref.1