Western Blot (WB)
(PIN4 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the PIN4 antibody detected the PIN4 protein (arrow).)
Rabbit anti-Human PIN4 Polyclonal Antibody | anti-PIN4 antibody
PIN4, NT (PIN4, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, Parvulin-14, Parvulin-17, Peptidyl-prolyl cis-trans isomerase Pin4, Peptidyl-prolyl cis/trans isomerase EPVH, Rotamase Pin4)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(PIN4 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the PIN4 antibody detected the PIN4 protein (arrow).)
Western Blot (WB)
(PIN4 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the PIN4 antibody detected the PIN4 protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. The encoded protein catalyzes the isomerization of peptidylprolyl bonds, and may play a role in the cell cycle, chromatin remodeling, and/or ribosome biogenesis. The encoded protein may play an additional role in the mitochondria. [provided by RefSeq, Dec 2009]
Uniprot Description
Function: Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA. Ref.10Isoform 2 binds to double-stranded DNA. Ref.10
Catalytic activity: Peptidylproline (omega=180) = peptidylproline (omega=0).
Subunit structure: Isoform 1 is found in pre-ribosomal ribonucleoprotein (pre-rRNP) complexes
By similarity. Ref.10
Subcellular location: Isoform 1: Nucleus › nucleolus. Cytoplasm › cytoskeleton › spindle. Cytoplasm. Note: Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1. Ref.1 Ref.2 Ref.6 Ref.7 Ref.9 Ref.10Isoform 2: Mitochondrion. Mitochondrion matrix. Note: Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide. Ref.1 Ref.2 Ref.6 Ref.7 Ref.9 Ref.10
Tissue specificity: Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much more abundant than isoform 2 in each tissue analyzed. Ref.1 Ref.2 Ref.5
Domain: The PPIase domain enhances mitochondrial targeting.
Post-translational modification: Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus. Ref.7 Ref.10Isoform 2 is sumoylated with SUMO2 and SUMO3. Ref.5
Sequence similarities: Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily.Contains 1 PpiC domain.
Sequence caution: The sequence AAH05234.2 differs from that shown. Reason: Erroneous initiation. The sequence AAH70288.1 differs from that shown. Reason: Erroneous initiation. The sequence AAI04654.1 differs from that shown. Reason: Erroneous initiation. The sequence AAI11395.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAG54532.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Research Articles on PIN4
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Product Notes
The PIN4 pin4 (Catalog #AAA648163) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIN4, NT (PIN4, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, Parvulin-14, Parvulin-17, Peptidyl-prolyl cis-trans isomerase Pin4, Peptidyl-prolyl cis/trans isomerase EPVH, Rotamase Pin4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIN4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the PIN4 pin4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIN4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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