Rabbit anti-Human ICOS Polyclonal Antibody | anti-ICOS antibody

ICOS, CT (Inducible T cell Co-stimulator, Activation Inducible Lymphocyte Immunomediatory Molecule, AILIM, CD278, CD278 Antigen, MGC39850) (FITC)

Cat. Num. AAA6481374

• Gene Names: ICOS; AILIM; CD278; CVID1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: ICOS; Polyclonal Antibody; CT (Inducible T cell Co-stimulator; Activation Inducible Lymphocyte Immunomediatory Molecule; AILIM; CD278; CD278 Antigen; MGC39850) (FITC); Inducible T-cell Costimulator; anti-ICOS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ICOS.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ICOS antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human ICOS (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lymph with I0649-95C, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Western Blot (WB)

(Western Blot analysis of I0649-95C in Jurkat cell line lysates (35ug/lane). This demonstrates the ICOS antibody detected the ICOS protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells (right histogram) using I0649-95C compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ICOS antibody

Antibodies; CD Markers

References

1. Okamoto, N., et al., Biochem. Biophys. Res. Commun. 310(3):691-702 (2003). 2. Okamoto, T., et al., J. Rheumatol. 30(6):1157-1163 (2003). 3. Riley, J.L., et al., Proc. Natl. Acad. Sci. USA 99(18):11790-11795 (2002). 4. Haimila, K.E., et al., Immunogenetics 53(12):1028-1032 (2002). 5. Witsch, E.J., et al., Eur. J. Immunol. 32(9):2680-2686 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 15029518
• NCBI GeneID: 29851
• NCBI Accession #: NP_036224
• NCBI GenBank Nucleotide #: NM_012092.3
• UniProt Accession #: Q9Y6W8; Q8N6W8
• Molecular Weight: 40.8kDa (362aa) 40-57kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: inducible T-cell costimulator
• NCBI Official Synonym Full Names: inducible T cell costimulator
• NCBI Official Symbol: ICOS
• NCBI Official Synonym Symbols: AILIM; CD278; CVID1
• NCBI Protein Information: inducible T-cell costimulator
• UniProt Protein Name: Inducible T-cell costimulator
• Protein Family: ICOS ligand
• UniProt Gene Name: ICOS
• UniProt Synonym Gene Names: AILIM
• UniProt Entry Name: ICOS_HUMAN

NCBI Description

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

ICOS: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. Defects in ICOS are the cause of immunodeficiency common variable type 1 (CVID1). CVID1 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be lo. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane; external side of plasma membrane

Biological Process: cell-cell adhesion; T cell tolerance induction; T cell costimulation; immune response

Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 1