Rabbit anti-Human HNRPK Polyclonal Antibody | anti-HNRPK antibody

HNRPK, NT (Heterogeneous Nuclear Ribonucleoprotein K, Transformation Up-regulated Nuclear Protein, hnRNP K, TUNP, HNRNPK) (FITC)

Cat. Num. AAA6481033

• Gene Names: HNRNPK; AUKS; CSBP; TUNP; HNRPK
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: HNRPK; Polyclonal Antibody; NT (Heterogeneous Nuclear Ribonucleoprotein K; Transformation Up-regulated Nuclear Protein; hnRNP K; TUNP; HNRNPK) (FITC); anti-HNRPK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HNRPK.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HNRPK antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human HNRPK (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of anti-HNRPK Antibody (N-term) in 293 cell line lysates (35ug/lane). HNRPK(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-HNRPK Antibody (N-term) in mouse brain tissue lysates (35ug/lane). HNRPK(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-HNRPK antibody

Antibodies; RNA Processing

References

1. Hsieh T.-Y., Matsumoto M., Chou H.-C., Schneider R., Hwang S.B., Lee A.S., Lai M.M.C.J. Biol. Chem. 273:17651-17659(1998). 2. Kwiek N.C., Thacker D.F., Datto M.B., Megosh H.B., Cell. Signal. 18:1769-1778(2006). 3. Dejgaard K., Leffers H., Rasmussen H.H., Madsen P., J. Mol. Biol. 236:33-48(1994). 4. Hernaez B., Escribano J.M., Alonso C.FEBS Lett. 582:3275-3280(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 14165435
• NCBI GeneID: 3190
• NCBI Accession #: NP_112552
• NCBI GenBank Nucleotide #: NM_031262
• UniProt Accession #: P61978
• Molecular Weight: 51kDa
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein K isoform b
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein K
• NCBI Official Symbol: HNRNPK
• NCBI Official Synonym Symbols: AUKS; CSBP; TUNP; HNRPK
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein K
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein K
• UniProt Gene Name: HNRNPK
• UniProt Synonym Gene Names: HNRPK; hnRNP K; TUNP
• UniProt Entry Name: HNRPK_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP K: a heterogeneous nuclear ribonucleoprotein (hnRNP). One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; Spliceosome; RNA-binding

Chromosomal Location of Human Ortholog: 9q21.32-q21.33

Cellular Component: nucleoplasm; cell projection; focal adhesion; membrane; cytoplasm; nuclear chromatin; podosome; nucleus

Molecular Function: protein binding; RNA binding; single-stranded DNA binding

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; viral reproduction; RNA splicing; positive regulation of low-density lipoprotein receptor biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; gene expression; signal transduction; positive regulation of receptor-mediated endocytosis