Rabbit anti-Human, Mouse GRK6 Polyclonal Antibody | anti-GRK6 antibody

GRK6, CT (G-Protein Coupled Receptor Kinase 6, G-Protein-coupled Receptor Kinase GRK6, GPRK6, FLJ32135) (Azide free) (HRP)

Cat. Num. AAA6479027

• Gene Names: GRK6; GPRK6
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: GRK6; Polyclonal Antibody; CT (G-Protein Coupled Receptor Kinase 6; G-Protein-coupled Receptor Kinase GRK6; GPRK6; FLJ32135) (Azide free) (HRP); EC=2.7.11.16; anti-GRK6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GRK6. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GRK6 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human GRK6 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-GRK6 Pab in HeLa cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2). GRK6 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(GRK6 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GRK6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-GRK6 antibody

Antibodies; G Protein Receptors

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Stoffel, R.H., et al., J. Biol. Chem. 269(45):27791-27794 (1994). 3. Benovic, J.L., et al., J. Biol. Chem. 268(26):19521-19527 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 51896037
• NCBI GeneID: 2870
• NCBI Accession #: NP_001004105.1
• NCBI GenBank Nucleotide #: NM_001004105.2
• UniProt Accession #: P43250; O60541; Q13652
• Molecular Weight: 65,991 Da
• NCBI Official Full Name: G protein-coupled receptor kinase 6 isoform C
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 6
• NCBI Official Symbol: GRK6
• NCBI Official Synonym Symbols: GPRK6
• NCBI Protein Information: G protein-coupled receptor kinase 6
• UniProt Protein Name: G protein-coupled receptor kinase 6
• Protein Family: G protein-coupled receptor kinase
• UniProt Gene Name: GRK6
• UniProt Synonym Gene Names: GPRK6
• UniProt Entry Name: GRK6_HUMAN

NCBI Description

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GRK6: Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamin receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro). Widely expressed. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.16; AGC group; GRK family; GRK subfamily

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: membrane

Molecular Function: G-protein coupled receptor kinase activity; protein binding; ATP binding

Biological Process: Wnt receptor signaling pathway; protein amino acid phosphorylation; regulation of G-protein coupled receptor protein signaling pathway