Western Blot (WB)
(Western Blot detection against Immunogen (36.74kD).)
Mouse anti-Human ALOX12 Monoclonal Antibody | anti-ALOX12 antibody
ALOX12 (Arachidonate 12-lipoxygenase, 12S-type, 12S-LOX, 12S-lipoxygenase, Platelet-type Lipoxygenase 12, LOG12)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot detection against Immunogen (36.74kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (36.74kD).)
Testing Data
(Detection limit for recombinant GST tagged ALOX12 is ~0.3ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged ALOX12 is ~0.3ng/ml as a capture antibody.)
NCBI and Uniprot Product Information
Uniprot Description
Function: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation. Ref.12 Ref.16 Ref.17 Ref.19 Ref.20
Catalytic activity: Arachidonate + O2 = (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate. Ref.11 Ref.12 Ref.13 Ref.14(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O = (5S,6R,15S)-trihydroxy-(7E,9E,11Z,13E)-eicosatetraenoate. Ref.11 Ref.12 Ref.13 Ref.14(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O = (5S,14R,15S)-trihydroxy-(6E,8Z,10E,12E)-eicosatetraenoate.
Cofactor: Binds 1 iron ion per subunit.
Enzyme regulation: Activated by EGF. Ref.15
Pathway: Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis. Ref.11
Subcellular location: Cytoplasm › cytosol. Membrane. Note: Membrane association is stimulated by EGF. Ref.13 Ref.15
Tissue specificity: Expressed in vascular smooth muscle cells. Ref.20
Induction: Down-regulated upon starvation, by UV-irradiation and 15-lipoxygenase metabolites. Ref.15 Ref.18
Involvement in disease: Esophageal cancer (ESCR) [MIM:133239]: A malignancy of the esophagus. The most common types are esophageal squamous cell carcinoma and adenocarcinoma. Cancer of the esophagus remains a devastating disease because it is usually not detected until it has progressed to an advanced incurable stage.Note: Disease susceptibility may be associated with variations affecting the gene represented in this entry. Gln at position 261 may confer interindividual susceptibility to esophageal cancer (Ref.24).Colorectal cancer (CRC) [MIM:114500]: A complex disease characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history.Note: Disease susceptibility may be associated with variations affecting the gene represented in this entry. Gln at position 261 may confer interindividual susceptibility to colorectal cancer (Ref.24).
Sequence similarities: Belongs to the lipoxygenase family.Contains 1 lipoxygenase domain.Contains 1 PLAT domain.
Biophysicochemical propertiesKinetic parameters:KM=8 µM for arachidonate (Ref.11, at pH 7.0 and 37 degrees Celsius) Ref.11 Ref.12 Ref.13KM=10 µM for arachidonate (Ref.13, at pH 8.0 and 25 degrees Celsius)KM=6.2 µM for arachidonate (Ref.12, at pH 7.4)KM=9 µM for linoleate (Ref.13, at pH 8.0 and 25 degrees Celsius)KM=7.9 µM for leukotriene A4 (Ref.12, at pH 7.4)KM=3 µM for eicosa-5,8,11,14,17-pentaenoate (Ref.11, at pH 7.0 and 37 degrees Celsius)KM=35 µM for eicosa-8,11,14-trienoate (Ref.11, at pH 7.0 and 37 degrees Celsius)KM=14.3 µM for 5,6-epoxy-8,11,14-eicosatrienoate (Ref.12, at pH 7.4)Vmax=3 µmol/min/mg enzyme with arachidonate as substrate (Ref.13, at pH 8.0 and 25 degrees Celsius)Vmax=1.057 µmol/min/mg enzyme with arachidonate as substrate (Ref.12, at pH 7.4)Vmax=0.0375 µmol/min/mg enzyme with linoleate as substrate (Ref.13, at pH 8.0 and 25 degrees Celsius)Vmax=0.025 µmol/min/mg enzyme with leukotriene A4 as substrate (Ref.12, at pH 7.4)Vmax=0.985 µmol/min/mg enzyme with 5,6-epoxy-8,11,14-eicosatrienoate as substrate (Ref.12, at pH 7.4)pH dependence:Optimum pH is 7.5-8.0 (for arachidonate 12-lipoxygenase activity).
Research Articles on ALOX12
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Product Notes
The ALOX12 alox12 (Catalog #AAA647798) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ALOX12 (Arachidonate 12-lipoxygenase, 12S-type, 12S-LOX, 12S-lipoxygenase, Platelet-type Lipoxygenase 12, LOG12) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALOX12 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the ALOX12 alox12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PPPTTKEDVT MATVMGSLPD VRQACLQMAI SWHLSRRQPD MVPLGHHKEK YFSGPKPKAV LNQFRTDLEK LEKEITARNE QLDWPYEYLK PSCIENSVTI. It is sometimes possible for the material contained within the vial of "ALOX12, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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