Rabbit anti-Human SIRT5 Polyclonal Antibody | anti-Sirt5 antibody

SIRT5, ID (SIRT5, SIR2L5, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2-like protein 5)

Cat. Num. AAA647773

• Gene Names: Sirt5; AV001953; 0610012J09Rik; 1500032M05Rik
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SIRT5; Polyclonal Antibody; ID (SIRT5; SIR2L5; NAD-dependent protein deacylase sirtuin-5; mitochondrial; Regulatory protein SIR2 homolog 5; SIR2-like protein 5); Anti -SIRT5; anti-Sirt5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-Sirt5 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: SIRT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-127 amino acids from the Central region of human SIRT5.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SIRT5 Antibody (Center) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the SIRT5 antibody detected the SIRT5 protein (arrow).)

Western Blot (WB)

(Western blot analysis of SIRT5 (arrow) using rabbit polyclonal SIRT5 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SIRT5 gene.)

Related Product Information for anti-Sirt5 antibody

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants.

Product Categories/Family for anti-Sirt5 antibody

Antibodies; Abs to Mitochondrial Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 56789008
• NCBI GeneID: 68346
• UniProt Accession #: Q8K2C6
• Molecular Weight: 34,134 Da
• NCBI Official Full Name: Sirt5 protein
• NCBI Official Synonym Full Names: sirtuin 5
• NCBI Official Symbol: Sirt5
• NCBI Official Synonym Symbols: AV001953; 0610012J09Rik; 1500032M05Rik
• NCBI Protein Information: NAD-dependent protein deacylase sirtuin-5, mitochondrial; SIR2-like protein 5; regulatory protein SIR2 homolog 5; NAD-dependent deacetylase sirtuin-5; NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial
• UniProt Protein Name: NAD-dependent protein deacylase sirtuin-5, mitochondrial
• Protein Family: NAD-dependent protein deacylase sirtuin
• UniProt Gene Name: Sirt5
• UniProt Synonym Gene Names: Sir2l5
• UniProt Entry Name: SIR5_MOUSE

Uniprot Description

Function: NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro. Ref.2 Ref.3 Ref.4

Catalytic activity: NAD+ + a malonylprotein = nicotinamide + O-malonyl-ADP-ribose + a protein. HAMAP-Rule MF_03160NAD+ + a succinylprotein = nicotinamide + O-succinyl-ADP-ribose + a protein. HAMAP-Rule MF_03160

Cofactor: Binds 1 zinc ion per subunit

By similarity. HAMAP-Rule MF_03160

Subunit structure: Monomer. Homodimer. Interacts with CPS1. Ref.2

Subcellular location: Mitochondrion Ref.2.

Tissue specificity: Detected in brain, liver, heart, kidney, lung, thymus, spleen, skeletal muscle, intestine, pancreas and testis (at protein level). Ref.2

Domain: In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity

By similarity. HAMAP-Rule MF_03160

Disruption phenotype: No visible phenotype. After 48 hours of fasting, the absence of Cps1 activation leads to elevated blood ammonia levels, possibly due to the presence of succinylation at 'Lys-1291' in Cps1. Ref.2 Ref.3 Ref.4

Sequence similarities: Belongs to the sirtuin family. Class III subfamily.Contains 1 deacetylase sirtuin-type domain.