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Western Blot (WB) (Western blot analysis of PFKM polyclonal antibody in Hela cell line lysate. PFKM(arrow) was detected using the purified Pab.)

Rabbit anti-Human PFKM Polyclonal Antibody | anti-PFKM antibody

PFKM, CT (6-phosphofructokinase, Muscle Type, Phosphofructokinase 1, Phosphohexokinase, Phosphofructo-1-kinase Isozyme A, PFK-A, Phosphofructokinase-M, PFKX) (AP)

Gene Names
PFKM; GSD7; PFK1; PFKA; PFKX; PFK-1; PFK-A; ATP-PFK; PPP1R122
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
PFKM; Polyclonal Antibody; CT (6-phosphofructokinase; Muscle Type; Phosphofructokinase 1; Phosphohexokinase; Phosphofructo-1-kinase Isozyme A; PFK-A; Phosphofructokinase-M; PFKX) (AP); anti-PFKM antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PFKM.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-PFKM antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human PFKM (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PFKM polyclonal antibody in Hela cell line lysate. PFKM(arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of PFKM polyclonal antibody in Hela cell line lysate. PFKM(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKM antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKM antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of Fructose 6 Phosphate Kinase (PFKM) Antibody(C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of Fructose 6 Phosphate Kinase (PFKM) Antibody(C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Product Categories/Family for anti-PFKM antibody
References
1. Howard, T.D., et al., Genomics 34(1):122-127 (1996). 2. Vasconcelos, O., et al., Proc. Natl. Acad. Sci. USA 92(22):10322-10326 (1995). 3. Raben, N., et al., J. Biol. Chem. 268(7):4963-4967 (1993). 4. Yamasaki, T., et al., Gene 104(2):277-282 (1991). 5. Sharma, P.M., et al., J. Biol. Chem. 265(16):9006-9010 (1990).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
93,254 Da
NCBI Official Full Name
ATP-dependent 6-phosphofructokinase, muscle type isoform 2
NCBI Official Synonym Full Names
phosphofructokinase, muscle
NCBI Official Symbol
PFKM
NCBI Official Synonym Symbols
GSD7; PFK1; PFKA; PFKX; PFK-1; PFK-A; ATP-PFK; PPP1R122
NCBI Protein Information
ATP-dependent 6-phosphofructokinase, muscle type
UniProt Protein Name
6-phosphofructokinase, muscle type
UniProt Gene Name
PFKM
UniProt Synonym Gene Names
PFKX; PFK-A
UniProt Entry Name
K6PF_HUMAN

NCBI Description

Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]

Research Articles on PFKM

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Product Notes

The PFKM pfkm (Catalog #AAA6477040) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PFKM, CT (6-phosphofructokinase, Muscle Type, Phosphofructokinase 1, Phosphohexokinase, Phosphofructo-1-kinase Isozyme A, PFK-A, Phosphofructokinase-M, PFKX) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PFKM can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PFKM pfkm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFKM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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