Rabbit anti-Human, Mouse FGG Polyclonal Antibody | anti-FGG antibody

FGG, NT (Fibrinogen gamma chain, PRO2061, FGG) (AP)

Cat. Num. AAA6476145

• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: FGG; Polyclonal Antibody; NT (Fibrinogen gamma chain; PRO2061; FGG) (AP); anti-FGG antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FGG. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
• Sequence Length: 453

• Applicable Applications for anti-FGG antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human FGG (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FGG Antibody (N-term) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the FGG antibody detected the FGG protein (arrow).)

Flow Cytometry (FC/FACS)

(FGG Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of FGG Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-FGG antibody

Antibodies; Coagulation Factors

References

1. Sie, M.P., Isaacs, A. J. Hypertens. 27(7), 1392-1398 (2009). 2. Nowak-Gottl, U., Weiler, H. Blood (2009). In press. 3. de Willige, S.U., Pyle, M.E. Thromb. Haemost. 101(6), 1078-1084 (2009). 4. Yoshida, N., Imaoka, S. Thromb. Haemost. 68(5), 534-538 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 70906439
• NCBI GeneID: 2266
• NCBI Accession #: NP_068656
• NCBI GenBank Nucleotide #: NP_068656.2
• UniProt Accession #: P02679
• Molecular Weight: 51.512
• NCBI Official Full Name: fibrinogen gamma chain isoform gamma-B
• NCBI Official Synonym Full Names: fibrinogen gamma chain
• NCBI Official Symbol: FGG
• NCBI Protein Information: fibrinogen gamma chain
• UniProt Protein Name: Fibrinogen gamma chain
• Protein Family: Fibrinogen
• UniProt Gene Name: FGG
• UniProt Entry Name: FIBG_HUMAN

NCBI Description

The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

Uniprot Description

FGG: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. Defects in FGG are a cause of congenital afibrinogenemia (CAFBN). This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q28

Cellular Component: cell surface; external side of plasma membrane; extracellular region; extracellular space; fibrinogen complex; plasma membrane

Molecular Function: cell adhesion molecule binding; protein binding; receptor binding; structural molecule activity

Biological Process: blood coagulation; cell-matrix adhesion; cellular protein complex assembly; extracellular matrix organization and biogenesis; fibrinolysis; plasminogen activation; platelet degranulation; positive regulation of exocytosis; positive regulation of heterotypic cell-cell adhesion; positive regulation of protein secretion; positive regulation of vasoconstriction; protein polymerization; protein secretion; response to calcium ion

Disease: Afibrinogenemia, Congenital; Dysfibrinogenemia, Congenital