Rabbit anti-Human EIF4E Polyclonal Antibody | anti-EIF4E antibody

EIF4E, Phosphorylated (Ser209) (Eukaryotic Translation Initiation Factor 4E, eIF4E, eIF-4E, mRNA Cap-binding Protein, eIF-4F 25kD Subunit, EIF4EL1, EIF4F) (FITC)

Cat. Num. AAA6475311

• Gene Names: EIF4E; CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: EIF4E; Polyclonal Antibody; Phosphorylated (Ser209) (Eukaryotic Translation Initiation Factor 4E; eIF4E; eIF-4E; mRNA Cap-binding Protein; eIF-4F 25kD Subunit; EIF4EL1; EIF4F) (FITC); anti-EIF4E antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EIF4E when phosphorylated at Ser209.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-EIF4E antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser209 of human EIF4E (KLH).
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-EIF4E-pS209 Phospho-specific Pab (RB13353) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-EIF4E antibody

Antibodies; Translation Initiation Factors

References

1. Rychlik, W., J. Biol. Chem. 262(22), 10434-10437 (1987). 2. Dorfman, J., Genomics 9(4), 785-788 (1991). 3. Pelletier, J., Genomics 10(4), 1079-1082 (1991). 4. Whalen, S.G., J. Biol. Chem. 271(20), 11831-11837 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 4503535
• NCBI GeneID: 1977
• NCBI Accession #: NP_001959
• NCBI GenBank Nucleotide #: NM_001968.4
• UniProt Accession #: P06730; Q96E95; B7Z6V1; D6RCQ6
• Molecular Weight: 27.2 kDa (237aa), confirmed by MALDI-TOF
• NCBI Official Full Name: eukaryotic translation initiation factor 4E isoform 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4E
• NCBI Official Symbol: EIF4E
• NCBI Official Synonym Symbols: CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
• NCBI Protein Information: eukaryotic translation initiation factor 4E
• UniProt Protein Name: Eukaryotic translation initiation factor 4E
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF4E
• UniProt Synonym Gene Names: EIF4EL1; EIF4F; eIF-4E; eIF4E
• UniProt Entry Name: IF4E_HUMAN

NCBI Description

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

EIF4E: a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the EIF4F complex.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: mRNA cap complex; stress granule; cytoplasm; eukaryotic translation initiation factor 4F complex; cytosol

Molecular Function: protein binding; eukaryotic initiation factor 4G binding; translation initiation factor activity; RNA cap binding

Biological Process: translation; viral reproduction; cytokine and chemokine mediated signaling pathway; positive regulation of mitotic cell cycle; regulation of translation; poly(A) tail shortening; mRNA export from nucleus; cellular protein metabolic process; insulin receptor signaling pathway; translational initiation; gene expression; mRNA catabolic process, deadenylation-dependent decay; lung development; G1/S transition of mitotic cell cycle

Disease: Autism, Susceptibility To, 19