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Western Blot (WB) (Western blot analysis of anti-EPRS Pab in Jurkat cell line lysates (35ug/lane). EPRS (arrow) was detected using the purified Pab.)

Rabbit anti-Human EPRS Polyclonal Antibody | anti-EPRS antibody

EPRS, NT (Bifunctional aminoacyl-tRNA Synthetase, Cell Proliferation-inducing Gene 32 Protein, Glutamyl-tRNA Synthetase, Glutamate-tRNA Ligase, GluRS, Prolyl-tRNA Synthetase, Proline-tRNA Ligase, GLNS, PARS, QARS, QPRS, PIG32) (HRP)

Gene Names
EPRS; EARS; PARS; QARS; QPRS; HLD15; PIG32; GLUPRORS
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
EPRS; Polyclonal Antibody; NT (Bifunctional aminoacyl-tRNA Synthetase; Cell Proliferation-inducing Gene 32 Protein; Glutamyl-tRNA Synthetase; Glutamate-tRNA Ligase; GluRS; Prolyl-tRNA Synthetase; Proline-tRNA Ligase; GLNS; PARS; QARS; QPRS; PIG32) (HRP); anti-EPRS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human EPRS.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-EPRS antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-1:50
ELISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human EPRS (KLH).
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of anti-EPRS Pab in Jurkat cell line lysates (35ug/lane). EPRS (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-EPRS Pab in Jurkat cell line lysates (35ug/lane). EPRS (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EPRS antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EPRS antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Product Categories/Family for anti-EPRS antibody
References
1. Jia, J., Mol. Cell 29(6), 679-690 (2008). 2. Beausoleil, S.A., Nat. Biotechnol. 24(10), 1285-1292 (2006). 3. Kato, T., Cancer Res. 65(13), 5638-5646 (2005). 4. Sampath, P., Cell 119(2), 195-208 (2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
170kDa
NCBI Official Full Name
bifunctional glutamate/proline--tRNA ligase
NCBI Official Synonym Full Names
glutamyl-prolyl-tRNA synthetase
NCBI Official Symbol
EPRS
NCBI Official Synonym Symbols
EARS; PARS; QARS; QPRS; HLD15; PIG32; GLUPRORS
NCBI Protein Information
bifunctional glutamate/proline--tRNA ligase
UniProt Protein Name
Bifunctional glutamate/proline--tRNA ligase
UniProt Gene Name
EPRS
UniProt Synonym Gene Names
GLNS; PARS; QARS; QPRS; GluRS

NCBI Description

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the multisynthetase complex and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Research Articles on EPRS

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Product Notes

The EPRS eprs (Catalog #AAA6474656) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EPRS, NT (Bifunctional aminoacyl-tRNA Synthetase, Cell Proliferation-inducing Gene 32 Protein, Glutamyl-tRNA Synthetase, Glutamate-tRNA Ligase, GluRS, Prolyl-tRNA Synthetase, Proline-tRNA Ligase, GLNS, PARS, QARS, QPRS, PIG32) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EPRS can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-1:50 ELISA: 1:1,000 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the EPRS eprs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPRS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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