Rabbit anti-Human EPS15R Polyclonal Antibody | anti-EPS15R antibody

EPS15R, NT (Epidermal Growth Factor Receptor Substrate 15-like 1, Eps15-related Protein, EPS15L1) (FITC)

Cat. Num. AAA6474622

• Gene Names: EPS15L1; EPS15R
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: EPS15R; Polyclonal Antibody; NT (Epidermal Growth Factor Receptor Substrate 15-like 1; Eps15-related Protein; EPS15L1) (FITC); anti-EPS15R antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EPS15R.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-EPS15R antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human EPS15R (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-EPS15R Pab is used in Western blot to detect EPS15R in Y79 cell lysate.)

Product Categories/Family for anti-EPS15R antibody

Antibodies; Receptors

References

1. J Biol Chem. 2002 Aug 23;277(34):30746-53. 2. J Biol Chem. 2002 Mar 15;277(11):8941-8. 3. J Biol Chem. 1998 Jan 30;273(5):3003-12. 4. Cancer Res. 1997 Dec 15;57(24):5498-504. 5. J Biol Chem. 1995 Jun 23;270(25):15341-7.

NCBI and Uniprot Product Information

• NCBI GI #: 10864047
• NCBI GeneID: 58513
• NCBI Accession #: NP_067058
• NCBI GenBank Nucleotide #: NM_021235
• UniProt Accession #: Q9UBC2
• Molecular Weight: 95kDa
• NCBI Official Full Name: epidermal growth factor receptor substrate 15-like 1 isoform 2
• NCBI Official Synonym Full Names: epidermal growth factor receptor pathway substrate 15 like 1
• NCBI Official Symbol: EPS15L1
• NCBI Official Synonym Symbols: EPS15R
• NCBI Protein Information: epidermal growth factor receptor substrate 15-like 1
• UniProt Protein Name: Epidermal growth factor receptor substrate 15-like 1
• UniProt Gene Name: EPS15L1
• UniProt Synonym Gene Names: EPS15R; Eps15R
• UniProt Entry Name: EP15R_HUMAN

Uniprot Description

EPS15R: epidermal growth factor receptor substrate protein 15R. Contains three EH domains which are implicated in endocytosis, vesicle transport and signal transduction. All three EH domains contain calcium-binding domains ofthe EF-hand type. Contains 2 UIM motifs, which are is responsible for ubiquitin recognition and monoubiquitination. May function as an adaptor between ubiquitinated proteins and the clathrin coat or other endocytic scaffolds.

Protein type: Adaptor/scaffold; Vesicle

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: membrane; plasma membrane; coated pit; nucleus

Molecular Function: protein binding; calcium ion binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; endocytosis