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Western Blot (WB) (Western blot analysis of ELOVL6 antibody (N-term) in mouse liver tissue lysates (35ug/lane). ELOVL6 (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse ELOVL6 Polyclonal Antibody | anti-ELOVL6 antibody

ELOVL6, NT (Elongation of Very Long Chain Fatty Acids Protein 6, Fatty Acid Elongase 2, hELO2, Fatty Acyl-CoA Elongase, Long-chain Fatty-acyl Elongase, LCE, FACE) (MaxLight 650)

Gene Names
ELOVL6; FAE; LCE; FACE
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
ELOVL6; Polyclonal Antibody; NT (Elongation of Very Long Chain Fatty Acids Protein 6; Fatty Acid Elongase 2; hELO2; Fatty Acyl-CoA Elongase; Long-chain Fatty-acyl Elongase; LCE; FACE) (MaxLight 650); anti-ELOVL6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ELOVL6. Species Crossreactivity: mouse.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Applicable Applications for anti-ELOVL6 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human ELOVL6 (KLH).
Conjugate
MaxLight650
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ELOVL6 antibody (N-term) in mouse liver tissue lysates (35ug/lane). ELOVL6 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of ELOVL6 antibody (N-term) in mouse liver tissue lysates (35ug/lane). ELOVL6 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ELOVL6 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ELOVL6 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ELOVL6 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (ELOVL6 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-ELOVL6 antibody
References
1. Lu, Y., J. Lipid Res. 49(12), 2582-2589 (2008).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,376 Da
NCBI Official Full Name
elongation of very long chain fatty acids protein 6
NCBI Official Synonym Full Names
ELOVL fatty acid elongase 6
NCBI Official Symbol
ELOVL6
NCBI Official Synonym Symbols
FAE; LCE; FACE
NCBI Protein Information
elongation of very long chain fatty acids protein 6; hELO2; ELOVL FA elongase 6; fatty acid elongase 2; fatty acyl-CoA elongase; long-chain fatty-acyl elongase; 3-keto acyl-CoA synthase ELOVL6; very-long-chain 3-oxoacyl-CoA synthase 6; ELOVL family member
UniProt Protein Name
Elongation of very long chain fatty acids protein 6
UniProt Gene Name
ELOVL6
UniProt Synonym Gene Names
FACE; LCE; ELOVL FA elongase 6; hELO2
UniProt Entry Name
ELOV6_HUMAN

NCBI Description

Fatty acid elongases (EC 6.2.1.3), such as ELOVL6, use malonyl-CoA as a 2-carbon donor in the first and rate-limiting step of fatty acid elongation (Moon et al., 2001 [PubMed 11567032]).[supplied by OMIM, Mar 2008]

Uniprot Description

ELOVL6: Condensing enzyme that catalyzes the synthesis of saturated and monounsaturated fatty acids. Highest activity toward C16:0 acyl-CoAs. Belongs to the ELO family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; EC 2.3.1.199

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane

Molecular Function: protein binding; transferase activity, transferring groups other than amino-acyl groups

Biological Process: long-chain fatty acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; fatty acid elongation, saturated fatty acid

Research Articles on ELOVL6

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Product Notes

The ELOVL6 elovl6 (Catalog #AAA6473769) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ELOVL6, NT (Elongation of Very Long Chain Fatty Acids Protein 6, Fatty Acid Elongase 2, hELO2, Fatty Acyl-CoA Elongase, Long-chain Fatty-acyl Elongase, LCE, FACE) (MaxLight 650) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ELOVL6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ELOVL6 elovl6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ELOVL6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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