Rabbit anti-Human EDA Polyclonal Antibody | anti-EDA antibody

EDA, NT (Ectodysplasin-A, Ectodermal Dysplasia Protein, EDA Protein, ED1, EDA2) (AP)

Cat. Num. AAA6473475

• Gene Names: EDA; ED1; HED; EDA1; EDA2; HED1; ODT1; XHED; ECTD1; XLHED; ED1-A1; ED1-A2; EDA-A1; EDA-A2; STHAGX1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: EDA; Polyclonal Antibody; NT (Ectodysplasin-A; Ectodermal Dysplasia Protein; EDA Protein; ED1; EDA2) (AP); anti-EDA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EDA.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-EDA antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human EDA (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-EDA Antibody (N-term) (RB13883) in Hela cell line lysates (35ug/lane). EDA(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EDA antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of hela cells using EDA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-EDA antibody

Antibodies; Disease Markers

References

1. Tariq, M., Eur J Dermatol 17(3), 209-212 (2007). 2. Tarpey, P., Am. J. Med. Genet. A 143(4), 390-394 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4503449
• NCBI GeneID: 1896
• NCBI Accession #: NP_001390
• NCBI GenBank Nucleotide #: NM_001399.4
• UniProt Accession #: Q92838; O75910; Q5JS00; Q5JUM7; Q9UP77; Q9Y6L0; Q9Y6L1; Q9Y6L2; A0AUZ2; A2A337; B7ZLU2; B7ZLU4
• Molecular Weight: Predicted: 16; 43 kDa; ; Observed: 39 kDa
• NCBI Official Full Name: ectodysplasin-A isoform 1
• NCBI Official Synonym Full Names: ectodysplasin A
• NCBI Official Symbol: EDA
• NCBI Official Synonym Symbols: ED1; HED; EDA1; EDA2; HED1; ODT1; XHED; ECTD1; XLHED; ED1-A1; ED1-A2; EDA-A1; EDA-A2; STHAGX1
• NCBI Protein Information: ectodysplasin-A; oligodontia 1; X-linked anhidroitic ectodermal dysplasia protein
• UniProt Protein Name: Ectodysplasin-A
• Protein Family: Ectodysplasin
• UniProt Gene Name: EDA
• UniProt Synonym Gene Names: ED1; EDA2; EDA protein
• UniProt Entry Name: EDA_HUMAN

NCBI Description

The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

EDA: Seems to be involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Isoform 1 binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor XEDAR. Defects in EDA are the cause of ectodermal dysplasia type 1 (ED1); also known as Christ-Siemens-Touraine syndrome or X-linked hypohidrotic ectodermal dysplasia (XLHED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ED1 is a disease characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. ED1 is the most common form of over 150 clinically distinct ectodermal dysplasias. Defects in EDA are the cause of tooth agenesis selective X-linked type 1 (STHAGX1). A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth). Belongs to the tumor necrosis factor family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq12-q13.1

Cellular Component: endoplasmic reticulum membrane; cytoskeleton; collagen; membrane; intracellular membrane-bound organelle; apical part of cell; integral to plasma membrane; extracellular region; integral to membrane; plasma membrane

Molecular Function: protein binding; tumor necrosis factor receptor binding; receptor binding

Biological Process: pigmentation; cell-matrix adhesion; ectoderm development; immune response; positive regulation of NF-kappaB import into nucleus; gene expression; signal transduction; cell differentiation; activation of NF-kappaB transcription factor; odontogenesis of dentine-containing teeth

Disease: Tooth Agenesis, Selective, X-linked, 1; Ectodermal Dysplasia 1, Hypohidrotic, X-linked