Goat ARAP1 Polyclonal Antibody | anti-ARAP1 antibody

ARAP1 (Arf-GAP with Rho-GAP Domain, ANK Repeat and PH Domain-containing Protein 1, Centaurin-delta-2, Cnt-d2, CENTD2, KIAA0782)

Cat. Num. AAA647344

• Gene Names: ARAP1; CENTD2
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ARAP1; Polyclonal Antibody; ARAP1 (Arf-GAP with Rho-GAP Domain; ANK Repeat and PH Domain-containing Protein 1; Centaurin-delta-2; Cnt-d2; CENTD2; KIAA0782); Anti -ARAP1 (Arf-GAP with Rho-GAP Domain; anti-ARAP1 antibody

• Host: Goat
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Clonality: Polyclonal
• Specificity: Recognizes mouse ARAP1. Species sequence homology: Bovine, canine, human, porcine and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-ARAP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunocytochemistry.; Dilution: ELISA: 1:32,000
• Immunogen: Synthetic peptide corresponding to C-AFTADPLSLLRNV, from mouse ARAP1, at C-terminal (NP_056057.2, NP_001035207.1, NP_001128662.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-ARAP1 antibody

ARAP1 is a 1450aa containing signaling protein, belonging to the centaurin family with SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, ARAP1 displays RHO-GAP and phosphatidylinositol (3, 4, 5) trisphosphate (PIP3)-dependent ARF-GAP activity that regulates ARF-, RHO-, and CDC42-dependent cell activities. It associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thus involved in both signal transduction and cellular trafficking transport of a receptor protein involved in apoptosis. This protein is likely involved in the regulation of the cell-specific trafficking of TNFRSF10A and might thus affect the efficacy of TRAIL-induced apoptosis. ARAP1, an adaptor protein, is ubiquitously expressed in most of the tissues.

Product Categories/Family for anti-ARAP1 antibody

Antibodies; Abs to Golgi Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 92091600
• NCBI GeneID: 116985
• NCBI Accession #: NP_001035207.1
• NCBI GenBank Nucleotide #: NM_001040118.2
• UniProt Accession #: Q96P48; O94879; Q4LDD5; Q59FI7; Q6PHS3; Q8WU51; Q96HP6; Q96L71; A3KLL7; B2RTS2
• Molecular Weight: 162,192 Da
• NCBI Official Full Name: arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform c
• NCBI Official Synonym Full Names: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
• NCBI Official Symbol: ARAP1
• NCBI Official Synonym Symbols: CENTD2
• NCBI Protein Information: arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1; cnt-d2; centaurin-delta-2; centaurin, delta 2; ARF-GAP, RHO-GAP, ankyrin repeat, and pleckstrin homology domains-containing protein 1
• UniProt Protein Name: Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
• Protein Family: Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein
• UniProt Gene Name: ARAP1
• UniProt Synonym Gene Names: CENTD2; KIAA0782; Cnt-d2
• UniProt Entry Name: ARAP1_HUMAN

NCBI Description

The protein encoded by this gene contains SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

Function: Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5

By similarity. Ref.1

Subunit structure: Interacts with TNFRSF10A. Ref.11

Subcellular location: Cytoplasm. Golgi apparatus › Golgi stack membrane; Peripheral membrane protein. Cell membrane. Note: Associated with Golgi stacks in resting cells. Throughout the cytoplasm and in surface protrusion in cells that are in the process of attaching to a surface and spreading. Ref.1 Ref.11

Tissue specificity: Detected in heart, skeletal muscle, spleen, kidney, liver, placenta, lung, peripheral blood leukocytes, adrenal gland, bone marrow, brain, lymph node, mammary gland, prostate, spinal cord, stomach, thyroid and trachea. Ref.1

Sequence similarities: Contains 1 Arf-GAP domain.Contains 4 PH domains.Contains 1 Ras-associating domain.Contains 1 Rho-GAP domain.Contains 1 SAM (sterile alpha motif) domain.

Sequence caution: The sequence BAA34502.2 differs from that shown. Reason: Erroneous initiation. The sequence BAD92710.1 differs from that shown. Reason: Erroneous initiation.