Rabbit anti-Human DCD Polyclonal Antibody | anti-DCD antibody

DCD, CT (Dermcidin, Preproteolysin, Survival-promoting Peptide, DCD-1, DSEP, AIDD) (APC)

Cat. Num. AAA6472296

• Gene Names: DCD; PIF; AIDD; DSEP; HCAP; DCD-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: DCD; Polyclonal Antibody; CT (Dermcidin; Preproteolysin; Survival-promoting Peptide; DCD-1; DSEP; AIDD) (APC); anti-DCD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DCD.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 110

• Applicable Applications for anti-DCD antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-1:50; IHC-P: 1:50-1:100; WB: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa74-103 in the C-terminal region of human DCD, UniProt Accession #NP_444513 Cellular Localization: Secreted
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DCD antibody (C-term) in T47D cell line lysates (35ug/lane). DCD (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(DCD Antibody (C-term) (RB18706) IHC analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DCD Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DCD Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-DCD antibody

Antibodies; Peptides

References

1. Todorov, P.T., J. Biol. Chem. 272(19): 12, 279-12, 288 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 16751921
• NCBI GeneID: 117159
• NCBI Accession #: NP_444513
• NCBI GenBank Nucleotide #: NP_444513.1
• UniProt Accession #: P81605
• Molecular Weight: 11.284
• NCBI Official Full Name: dermcidin isoform 1 preproprotein
• NCBI Official Synonym Full Names: dermcidin
• NCBI Official Symbol: DCD
• NCBI Official Synonym Symbols: PIF; AIDD; DSEP; HCAP; DCD-1
• NCBI Protein Information: dermcidin
• UniProt Protein Name: Dermcidin
• Protein Family: Dermcidin
• UniProt Gene Name: DCD
• UniProt Synonym Gene Names: AIDD; DSEP
• UniProt Entry Name: DCD_HUMAN

NCBI Description

This antimicrobial gene encodes a secreted protein that is subsequently processed into mature peptides of distinct biological activities. The C-terminal peptide is constitutively expressed in sweat and has antibacterial and antifungal activities. The N-terminal peptide, also known as diffusible survival evasion peptide, promotes neural cell survival under conditions of severe oxidative stress. A glycosylated form of the N-terminal peptide may be associated with cachexia (muscle wasting) in cancer patients. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Uniprot Description

DCD: DCD-1 displays antimicrobial activity thereby limiting skin infection by potential pathogens in the first few hours after bacterial colonization. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat. Also exhibits proteolytic activity. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; EC 3.4.-.-

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: extracellular region

Molecular Function: peptidase activity

Biological Process: killing of cells of another organism; defense response to bacterium; proteolysis; defense response to fungus