Rabbit anti-Human DGUOK Polyclonal Antibody | anti-DGUOK antibody

DGUOK, NT (Deoxyguanosine Kinase, Mitochondrial, dGK) (PE)

Cat. Num. AAA6472239

• Gene Names: DGUOK; dGK; NCPH; PEOB4; MTDPS3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: DGUOK; Polyclonal Antibody; NT (Deoxyguanosine Kinase; Mitochondrial; dGK) (PE); anti-DGUOK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DGUOK.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-DGUOK antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human DGUOK (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-hDGUOK-M1 Pab in 293 cell line lysate (35ug/lane). hDGUOK-M1(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-DGUOK Pab in mouse kidney tissue lysate (35ug/lane). DGUOK(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-DGUOK antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 18426967
• NCBI GeneID: 1716
• NCBI Accession #: NP_550438
• NCBI GenBank Nucleotide #: NM_080916
• UniProt Accession #: Q16854
• Molecular Weight: 28kDa
• NCBI Official Full Name: deoxyguanosine kinase, mitochondrial isoform a
• NCBI Official Synonym Full Names: deoxyguanosine kinase
• NCBI Official Symbol: DGUOK
• NCBI Official Synonym Symbols: dGK; NCPH; PEOB4; MTDPS3
• NCBI Protein Information: deoxyguanosine kinase, mitochondrial
• UniProt Protein Name: Deoxyguanosine kinase, mitochondrial
• UniProt Gene Name: DGUOK
• UniProt Synonym Gene Names: DGK; dGK
• UniProt Entry Name: DGUOK_HUMAN

NCBI Description

In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by this gene is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DGUOK: Required for the phosphorylation of several deoxyribonucleosides and certain nucleoside analogs widely employed as antiviral and chemotherapeutic agents. Defects in DGUOK are the cause of mitochondrial DNA depletion syndrome type 3 (MTDPS3). A disorder characterized by onset in infancy of progressive liver failure, hypoglycemia, increased lactate in body fluids, and neurologic abnormalities including hypotonia, encephalopathy, peripheral neuropathy. Affected tissues show both decreased activity of the mtDNA-encoded respiratory chain complexes and mtDNA depletion. Belongs to the DCK/DGK family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, other; Nucleotide Metabolism - purine; EC 2.7.1.113; Mitochondrial

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: mitochondrion; mitochondrial matrix; cytosol; nucleus

Molecular Function: nucleoside kinase activity; deoxyguanosine kinase activity; ATP binding

Biological Process: purine deoxyribonucleoside metabolic process; nucleotide biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; dGTP metabolic process; guanosine metabolic process; protein amino acid phosphorylation; purine base metabolic process; purine salvage; deoxyribonucleoside monophosphate biosynthetic process

Disease: Mitochondrial Dna Depletion Syndrome 3 (hepatocerebral Type)