Rabbit anti-Human C1QTNF1 Polyclonal Antibody | anti-C1QTNF1 antibody

C1QTNF1, NT (Complement C1q Tumor Necrosis Factor-related pRotein 1, G-Protein-coupled Receptor-interacting Protein, GIP, CTRP1, UNQ310/PRO353) (AP)

Cat. Num. AAA6470799

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: C1QTNF1; Polyclonal Antibody; NT (Complement C1q Tumor Necrosis Factor-related pRotein 1; G-Protein-coupled Receptor-interacting Protein; GIP; CTRP1; UNQ310/PRO353) (AP); anti-C1QTNF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human C1QTNF1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
• Sequence Length: 281

• Applicable Applications for anti-C1QTNF1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human C1QTNF1 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of C1QTNF1 antibody (N-term) in A2058 cell line lysates (35ug/lane). C1QTNF1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skin tissue reacted with C1QTNF1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of C1QTNF1 (arrow) using rabbit polyclonal C1QTNF1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the C1QTNF1 gene.)

Flow Cytometry (FC/FACS)

(C1QTNF1 Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-C1QTNF1 antibody

Antibodies; Obesity Proteins

References

1. Wong, G.W., Biochem. J. 416(2), 161-177 (2008). 2. Ennis, S., Lancet 372(9652), 1828-1834 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 13569944
• NCBI Accession #: NP_112230
• NCBI GenBank Nucleotide #: NP_112230.1
• UniProt Accession #: Q9BXJ1
• Molecular Weight: 31.743
• NCBI Official Full Name: complement C1q tumor necrosis factor-related protein 1 isoform 1
• UniProt Protein Name: Complement C1q tumor necrosis factor-related protein 1
• Protein Family: Complement C1q tumor necrosis factor-related protein
• UniProt Gene Name: C1QTNF1
• UniProt Synonym Gene Names: CTRP1; GIP
• UniProt Entry Name: C1QT1_HUMAN

Uniprot Description

C1QTNF1: 2 isoforms of the human protein are produced by alternative splicing

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: extracellular space; collagen; integral to plasma membrane

Molecular Function: collagen binding; protein binding

Biological Process: positive regulation of protein kinase B signaling cascade; elevation of cytosolic calcium ion concentration; positive regulation of MAPKKK cascade; protein homooligomerization