Western Blot (WB)
(Western blot analysis of MEKK8 (arrow) using MEKK8 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K8 gene (Lane 2) (Origene Technologies).)
Rabbit anti-Human COT Polyclonal Antibody | anti-COT antibody
COT, CT (Cancer Osaka Thyroid Oncogene, c-COT, EST, ESTF, Mitogen-activated Protein Kinase Kinase Kinase 8, MAP3K8, MEKK8, Proto-oncogene c-Cot, Serine/threonine-protein Kinase Cot, Tumor Progression Locus 2, TPL2, TPL-2) (APC)
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of MEKK8 (arrow) using MEKK8 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K8 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of MEKK8 (arrow) using MEKK8 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K8 gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
NCBI and Uniprot Product Information
NCBI Description
This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]
Uniprot Description
Cot: an oncogenic Ser/Thr kinase of the STE group. Activates IkappaB kinases, thus inducing the nuclear translocation of NF-kappaB. Promotes the production of TNF-alpha and IL-2 during T lymphocyte activation. Interacts with NFkB-p105. Overexpressed and amplified in breast tumors. Viral insertions induce rat lymphomas and mouse mammary carcinomas. Isolated as a transforming factor in two cell lines. Mediates LPS activation of macrophages. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Oncoprotein; EC 2.7.11.25; STE group; STE-Unique family
Chromosomal Location of Human Ortholog: 10p11.23
Cellular Component: cytoplasm; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity
Biological Process: activation of MAPKK activity; apoptosis; regulation of cell differentiation; T cell costimulation; MAPKKK cascade; cell cycle; protein amino acid phosphorylation
Disease: Lung Cancer
Research Articles on COT
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Product Notes
The COT map3k8 (Catalog #AAA6470346) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The COT, CT (Cancer Osaka Thyroid Oncogene, c-COT, EST, ESTF, Mitogen-activated Protein Kinase Kinase Kinase 8, MAP3K8, MEKK8, Proto-oncogene c-Cot, Serine/threonine-protein Kinase Cot, Tumor Progression Locus 2, TPL2, TPL-2) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COT can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the COT map3k8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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