Rabbit anti-Human COG4 Polyclonal Antibody | anti-COG4 antibody

COG4, CT (Component Of Oligomeric Golgi Complex 4, Conserved Oligomeric Golgi Complex Subunit 4, COG Complex Subunit 4) (MaxLight 650)

Cat. Num. AAA6469597

• Gene Names: COG4; COD1; CDG2J; SWILS
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: COG4; Polyclonal Antibody; CT (Component Of Oligomeric Golgi Complex 4; Conserved Oligomeric Golgi Complex Subunit 4; COG Complex Subunit 4) (MaxLight 650); anti-COG4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human COG4.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-COG4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human COG4 (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of COG4 Antibody (C-term) in A2058 cell line lysates (35ug/lane). COG4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle with COG4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of jurkat cells using COG4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-COG4 antibody

Antibodies; Golgi Proteins

References

1. Whyte J.R., Munro S.Dev. Cell 1:527-537(2001). 2. Ota T., Suzuki Y., Nishikawa T.Nat. Genet. 36:40-45(2004). 3. Rikova K., Guo A., Zeng Q.Cell 131:1190-1203(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 118498354
• NCBI GeneID: 25839
• NCBI Accession #: NP_056201
• NCBI GenBank Nucleotide #: NM_015386
• UniProt Accession #: Q9H9E3
• Molecular Weight: 89kDa
• NCBI Official Full Name: conserved oligomeric Golgi complex subunit 4 isoform 1
• NCBI Official Synonym Full Names: component of oligomeric golgi complex 4
• NCBI Official Symbol: COG4
• NCBI Official Synonym Symbols: COD1; CDG2J; SWILS
• NCBI Protein Information: conserved oligomeric Golgi complex subunit 4
• UniProt Protein Name: Conserved oligomeric Golgi complex subunit 4
• Protein Family: Conserved oligomeric Golgi complex
• UniProt Gene Name: COG4
• UniProt Synonym Gene Names: COG complex subunit 4
• UniProt Entry Name: COG4_HUMAN

NCBI Description

The protein encoded by this gene is a component of an oligomeric protein complex involved in the structure and function of the Golgi apparatus. Defects in this gene may be a cause of congenital disorder of glycosylation type IIj. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2010]

Uniprot Description

Function: Required for normal Golgi function. Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with SCFD1. Ref.9

Subunit structure: Monomer. Component of the conserved oligomeric Golgi (COG) complex which is composed of eight different subunits and is required for normal Golgi morphology and localization. Mediates interaction of SCFD1 with the COG complex. Interacts with STX5. Ref.9 Ref.11

Subcellular location: Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side

Probable Ref.6.

Involvement in disease: Congenital disorder of glycosylation 2J (CDG2J) [MIM:613489]: A multisystem disorder caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.11 Ref.12

Sequence similarities: Belongs to the COG4 family.

Sequence caution: The sequence BAB15483.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.