Rabbit anti-Human CLOCK Polyclonal Antibody | anti-CLOCK antibody

CLOCK, ID (Circadian Locomoter Output Cycles Protein Kaput, hCLOCK, Class E Basic Helix-loop-helix Protein 8, BHLHE8, KIAA0334) (APC)

Cat. Num. AAA6469528

• Gene Names: CLOCK; KAT13D; bHLHe8
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CLOCK; Polyclonal Antibody; ID (Circadian Locomoter Output Cycles Protein Kaput; hCLOCK; Class E Basic Helix-loop-helix Protein 8; BHLHE8; KIAA0334) (APC); anti-CLOCK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CLOCK.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CLOCK antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CLOCK (KLH).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CLOCK Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). CLOCK (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with CLOCK antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-CLOCK antibody

Antibodies; Circadian Proteins

References

1. Sookoian, S., Am. J. Clin. Nutr. 87(6), 1606-1615 (2008). 2. Nakashima, A., Mol. Cell. Biol. 28(12), 4080-4092 (2008). 3. Marino, J.L., Epidemiology 19(3), 477-484 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4758010
• NCBI GeneID: 9575
• NCBI Accession #: NP_004889
• NCBI GenBank Nucleotide #: NM_004898
• UniProt Accession #: O15516
• Molecular Weight: 95kDa
• NCBI Official Full Name: circadian locomoter output cycles protein kaput
• NCBI Official Synonym Full Names: clock circadian regulator
• NCBI Official Symbol: CLOCK
• NCBI Official Synonym Symbols: KAT13D; bHLHe8
• NCBI Protein Information: circadian locomoter output cycles protein kaput
• UniProt Protein Name: Circadian locomoter output cycles protein kaput
• Protein Family: Clock-controlled protein
• UniProt Gene Name: CLOCK
• UniProt Synonym Gene Names: BHLHE8; KIAA0334; hCLOCK; bHLHe8
• UniProt Entry Name: CLOCK_HUMAN

NCBI Description

The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

CLOCK: ARNTL/2-CLOCK heterodimers activate E-box element (3'- CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression. Acetylates primarily histones H3 and H4. Acetylates also a non-histone substrate: ARNTL. Plays a role in DNA damage response (DDR) signaling during the S phase. Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with ARNTL is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and ARNTL. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-ARNTL heterodimer with PER or CRY inhibits transcription activation. Binds weakly ARNTL and ARNTL2 to form heterodimers which bind poorly to the E-box motif. Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).

Protein type: DNA-binding; Transcription factor; EC 2.3.1.48; Acetyltransferase

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: nucleoplasm; transcription factor complex; intracellular membrane-bound organelle; chromosome; cytosol; nucleus

Molecular Function: protein dimerization activity; signal transducer activity; protein binding; histone acetyltransferase activity; DNA binding; chromatin DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; establishment and/or maintenance of chromatin architecture; positive regulation of transcription, DNA-dependent; response to redox state; signal transduction; activation of NF-kappaB transcription factor; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; photoperiodism; DNA damage checkpoint; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; circadian regulation of gene expression; histone acetylation; negative regulation of transcription, DNA-dependent; regulation of hair cycle; regulation of insulin secretion; positive regulation of inflammatory response