Rabbit anti-Human Cathepsin B Polyclonal Antibody | anti-CATB antibody

Cathepsin B, ID (CATB, Cathepsin B1, APP Secretase, APPS, Cathepsin B Light Chain, Cathepsin B Heavy Chain, CPSB, CTSB) (Azide free) (HRP)

Cat. Num. AAA6467662

• Gene Names: CTSB; APPS; CPSB; RECEUP
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Cathepsin B; Polyclonal Antibody; ID (CATB; Cathepsin B1; APP Secretase; APPS; Cathepsin B Light Chain; Cathepsin B Heavy Chain; CPSB; CTSB) (Azide free) (HRP); anti-CATB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CATB.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CATB antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CATB (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CATB Antibody (Center) in Y79 cell line lysates (35ug/lane). CATB (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with CATB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using CATB Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CATB antibody

Antibodies; Cathepsin

References

1. Zhang, H., J. Immunol. 182(11), 6993-7000 (2009). 2. Duncan, J.A., J. Immunol. 182(10), 6460-6469 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503139
• NCBI GeneID: 1508
• NCBI Accession #: NP_001899; NP_680090; NP_680091; NP_680092; NP_680093
• NCBI GenBank Nucleotide #: NP_001899.1
• UniProt Accession #: P07858
• NCBI Official Full Name: cathepsin B isoform 1 preproprotein
• NCBI Official Synonym Full Names: cathepsin B
• NCBI Official Symbol: CTSB
• NCBI Official Synonym Symbols: APPS; CPSB; RECEUP
• NCBI Protein Information: cathepsin B
• UniProt Protein Name: Cathepsin B
• Protein Family: Catalase
• UniProt Gene Name: CTSB
• UniProt Synonym Gene Names: CPSB; APPS
• UniProt Entry Name: CATB_HUMAN

NCBI Description

This gene encodes a member of the C1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to form the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer's disease, the most common cause of dementia. Overexpression of the encoded protein has been associated with esophageal adenocarcinoma and other tumors. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Nov 2015]

Uniprot Description

CTSB: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Belongs to the peptidase C1 family.

Protein type: Autophagy; Motility/polarity/chemotaxis; EC 3.4.22.1; Protease

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: extracellular space; mitochondrion; intracellular membrane-bound organelle; perinuclear region of cytoplasm; lysosome; nucleolus; extracellular region; melanosome; intracellular

Molecular Function: collagen binding; peptidase activity; proteoglycan binding; protein binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: regulation of apoptosis; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; epithelial cell differentiation; proteolysis involved in cellular protein catabolic process; regulation of catalytic activity; toll-like receptor signaling pathway; innate immune response; decidualization; proteolysis