Rabbit anti-Human CPB2 Polyclonal Antibody | anti-CPB2 antibody

CPB2, ID (E134) (Carboxypeptidase B2, Carboxypeptidase U, CPU, Thrombin-activable Fibrinolysis Inhibitor, TAFI, Plasma Carboxypeptidase B, pCPB) (MaxLight 550)

Cat. Num. AAA6467093

• Gene Names: CPB2; CPU; PCPB; TAFI
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CPB2; Polyclonal Antibody; ID (E134) (Carboxypeptidase B2; Carboxypeptidase U; CPU; Thrombin-activable Fibrinolysis Inhibitor; TAFI; Plasma Carboxypeptidase B; pCPB) (MaxLight 550); anti-CPB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CPB2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-CPB2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CPB2 (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CPB2 antibody (Center E134) in Jurkat cell line lysates (35ug/lane). CPB2 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-CPB2 antibody

Antibodies; Enzymes

References

1. Valnickova, Z., J. Biol. Chem. 271(22), 12937-12943 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 513126885
• NCBI GeneID: 1361
• NCBI Accession #: NP_001863
• NCBI GenBank Nucleotide #: NM_001872
• UniProt Accession #: Q96IY4
• Molecular Weight: 36kDa
• NCBI Official Full Name: carboxypeptidase B2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase B2
• NCBI Official Symbol: CPB2
• NCBI Official Synonym Symbols: CPU; PCPB; TAFI
• NCBI Protein Information: carboxypeptidase B2
• UniProt Protein Name: Carboxypeptidase B2
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPB2
• UniProt Synonym Gene Names: CPU; pCPB; TAFI
• UniProt Entry Name: CBPB2_HUMAN

NCBI Description

Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Protease; Secreted, signal peptide; EC 3.4.17.20

Chromosomal Location of Human Ortholog: 13q14.11

Cellular Component: extracellular space

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: response to drug; fibrinolysis; negative regulation of fibrinolysis; response to heat; blood coagulation; proteolysis