Rabbit anti-Human CAMK1D Polyclonal Antibody | anti-CAMK1D antibody

CAMK1D, CT (Calcium/Calmodulin-dependent Protein Kinase Type 1D, CaM kinase ID, CaM Kinase I delta, CaMKI-delta, CaM-KI delta, CaMKI delta, Camk1D, CamKI-like Protein Kinase, CKLiK, CamKI-like Protein Kinase) (Biotin)

Cat. Num. AAA6466736

• Gene Names: CAMK1D; CKLiK; CaM-K1; CaMKID
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CAMK1D; Polyclonal Antibody; CT (Calcium/Calmodulin-dependent Protein Kinase Type 1D; CaM kinase ID; CaM Kinase I delta; CaMKI-delta; CaM-KI delta; CaMKI delta; Camk1D; CamKI-like Protein Kinase; CKLiK; CamKI-like Protein Kinase) (Biotin); anti-CAMK1D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CAMK1D.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CAMK1D antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CAMK1-like protein kinase (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CAMK1-like C-term Pab in NCIH460 cell lysate. CAMK1-like (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB)

(Western blot analysis of CAMK1D (arrow) using CAMK1D (CAMK1 delta)Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1D gene (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CAMK1-like Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-CAMK1D antibody

Antibodies; Calmodulin

References

1. Verploegen, S., et al., Blood 96(9):3215-3223 (2000). 2. Ishikawa, Y., et al., FEBS Lett. 550(1-3), 57-63 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 23943850
• NCBI GeneID: 57118
• NCBI Accession #: NP_705718
• NCBI GenBank Nucleotide #: NM_153498
• UniProt Accession #: Q8IU85; Q9HD31; B0YIY0
• Molecular Weight: 40,190 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type 1D isoform 2
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase ID
• NCBI Official Symbol: CAMK1D
• NCBI Official Synonym Symbols: CKLiK; CaM-K1; CaMKID
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type 1D; CaM kinase ID; CamKI-like protein kinase; caM kinase I delta; caM-KI delta; caMKI delta
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type 1D
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK1D
• UniProt Synonym Gene Names: CAMKID; CaM kinase ID; CaM-KI delta; CaMKI delta; CaMKID; CKLiK
• UniProt Entry Name: KCC1D_HUMAN

Uniprot Description

CAMK1D: a calcium/calmodulin-dependent S/T protein kinase of the CAMK group. Binding of calmodulin is thought to result in a conformational change and can lead to subsequent activation through phosphorylation by CAMKK1 and CAMKK2. Can be activated by CAMKK1 in a calcium-independent manner. Its Ca(2+)/CaM-dependent activity is enhanced 30-fold in vitro by phosphorylation at Thr180 by CaMKK1. Broadly expressed. Highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. May regulate calcium-mediated granulocyte function. May play a role in apoptosis of erythroleukemia cells. In vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1. Expression increases upon treatment of EC cells with DMSO and retinoic acid. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.17; CAMK group; CAMK1 family

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: cytoplasm; nucleus

Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: positive regulation of apoptosis; positive regulation of phagocytosis; activation of CREB transcription factor; regulation of dendrite development; inflammatory response; protein amino acid phosphorylation; negative regulation of apoptosis