Rabbit CAMK1 Polyclonal Antibody | anti-CAMK1 antibody

CAMK1, CT (Calcium/Calmodulin-dependent Protein Kinase Type 1, CaM Kinase I, CaM-KI, CaM Kinase I alpha, CaMKI-alpha) (FITC)

Cat. Num. AAA6466726

• Gene Names: CAMK1; CAMKI
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CAMK1; Polyclonal Antibody; CT (Calcium/Calmodulin-dependent Protein Kinase Type 1; CaM Kinase I; CaM-KI; CaM Kinase I alpha; CaMKI-alpha) (FITC); anti-CAMK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CAMK1. Species Crossreactivity: mouse, rat, primate.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CAMK1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CAMK1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-CAMK1 C-term Pab is used in Western blot to detect CAMK1 in HeLa cell lysate (lane 1) and primate brain tissue lysate (lane 2).)

Western Blot (WB)

(Western blot analysis of CAMK1 (arrow) using rabbit polyclonal CAMK1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-CAMK1 antibody

Antibodies; Calmodulin

References

1. Condon, J.C., et al., Endocrinology 143(9):3651-3657 (2002). 2. Chin, D., et al., J. Biol. Chem. 272(50):31235-31240 (1997). 3. Haribabu, B., et al., EMBO J. 14(15):3679-3686 (1995). 4. Ishikawa, Y., et al., FEBS Lett. 550(1-3), 57-63 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4502553
• NCBI GeneID: 8536
• NCBI Accession #: NP_003647.1
• NCBI GenBank Nucleotide #: NM_003656.4
• UniProt Accession #: Q14012; Q3KPF6
• Molecular Weight: 41,337 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type 1
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase I
• NCBI Official Symbol: CAMK1
• NCBI Official Synonym Symbols: CAMKI
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type 1; caM-KI; caMKI-alpha; caM kinase I alpha
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type 1
• Protein Family: Calcium/calmodulin-dependent serine/threonine-protein kinase
• UniProt Gene Name: CAMK1
• UniProt Synonym Gene Names: CaM-KI; CaMKI-alpha
• UniProt Entry Name: KCC1A_HUMAN

NCBI Description

Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008]

Uniprot Description

CAMK1A: an ubiquitous protein kinase of the CAMK1 family. Activated by Ca(2+)/calmodulin. Must be phosphorylated to be maximally active. Substrates include synapsin I.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.17; CAMK group; CAMK1 family

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cytoplasm; nucleus

Molecular Function: calmodulin binding; protein binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: positive regulation of syncytium formation by plasma membrane fusion; regulation of muscle cell differentiation; nucleocytoplasmic transport; signal transduction; cell cycle; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein export from nucleus; positive regulation of synapse structural plasticity; regulation of protein localization; regulation of protein binding; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein binding