Rabbit anti-Human BRK Polyclonal Antibody | anti-BRK antibody

BRK, NT (Protein-tyrosine Kinase 6, Breast Tumor Kinase, Tyrosine-protein Kinase BRK, PTK6) (PE)

Cat. Num. AAA6465994

• Gene Names: PTK6; BRK
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: BRK; Polyclonal Antibody; NT (Protein-tyrosine Kinase 6; Breast Tumor Kinase; Tyrosine-protein Kinase BRK; PTK6) (PE); anti-BRK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PTK6.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-BRK antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PTK6 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of SKBR3 lysate usingMBS613921. Band observed at ~52kD indicates Brk. An upper band is thought to be the 73kD ELF-related protein that shares 70% homology with the immunizing peptide.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-BRK antibody

Antibodies; Protein Kinases

References

1. Kasprzycka, Monika, et al (2006). Expression and oncogenic role of Brk (PTK6/Sik) protein tyrosine kinase in lymphocytes. Am J Pathol 168: 1631-41. 2. Haegebarth, Andrea, et al (2005). The intracellular tyrosine kinase Brk sensitizes non-transformed cells to inducers of apoptosis. Cell Cycle 4: 1239-46. 3. Qiu, Haoqun, et al (2005). Interaction between Brk kinase and insulin receptor substrate-4. Oncogene 24: 5656-64. 4. Qiu, Haoqun and Miller, W Todd (2002). Regulation of the nonreceptor tyrosine kinase Brk by autophosphorylation and by autoinhibition. J Biol Chem 277: 34634-41.

NCBI and Uniprot Product Information

• NCBI GI #: 373432692
• NCBI GeneID: 5753
• NCBI Accession #: NP_001243287.1
• NCBI GenBank Nucleotide #: NM_001256358.1
• UniProt Accession #: Q13882; Q58F01; B2RCR3; B4DW46
• Molecular Weight: 51,834 Da
• NCBI Official Full Name: protein-tyrosine kinase 6 isoform 2
• NCBI Official Synonym Full Names: protein tyrosine kinase 6
• NCBI Official Symbol: PTK6
• NCBI Official Synonym Symbols: BRK
• NCBI Protein Information: protein-tyrosine kinase 6; breast tumor kinase; protein-tyrosine kinase BRK; tyrosine-protein kinase BRK; PTK6 protein tyrosine kinase 6
• UniProt Protein Name: Protein-tyrosine kinase 6
• Protein Family: BrkA autotransporter
• UniProt Gene Name: PTK6
• UniProt Synonym Gene Names: BRK
• UniProt Entry Name: PTK6_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

Brk: a tyrosine kinase of the Src family. May function as an intracellular signal transducer in epithelial tissues. Very high level expression in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Selectively expressed in breast tumors and cell lines, and perhaps in colon and prostate cancers. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Overexpression in mammary cells leads to EGF sensitization and results in a partially transformed phenotype. Enhances anchorage-independent growth and responsiveness to EGF. RNAi reduces proliferation in breast cancer cells. Kinase-inactive mutant indicates tumor function may be independent of catalytic function.

Protein type: Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; TK group; Src family

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: nucleoplasm; ruffle; extrinsic to internal side of plasma membrane; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: cell migration; protein amino acid autophosphorylation; innate immune response; tyrosine phosphorylation of Stat3 protein; actin cytoskeleton organization and biogenesis; tyrosine phosphorylation of Stat5 protein; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation; negative regulation of growth