Rabbit anti-Human BRAF Polyclonal Antibody | anti-BRAF antibody

BRAF, Phosphorylated (Thr400) (Serine/Threonine-protein Kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf Murine Sarcoma Viral Oncogene Homolog B1, BRAF1, RAFB1) (Azide free) (HRP)

Cat. Num. AAA6465816

• Gene Names: BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BRAF; Polyclonal Antibody; Phosphorylated (Thr400) (Serine/Threonine-protein Kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf Murine Sarcoma Viral Oncogene Homolog B1; BRAF1; RAFB1) (Azide free) (HRP); anti-BRAF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BRAF when phosphorylated at Thr400.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BRAF antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr400 of human BRAF (KLH).
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-BRAF Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-BRAF antibody

Antibodies; Protein Kinases

References

1. Loewe, R., et al., J. Invest. Dermatol. 123(4):733-736 (2004). 2. Yamaguchi, T., et al., J. Biol. Chem. 279(39):40419-40430 (2004). 3. Frattini, M., et al., Oncogene 23(44):7436-7440 (2004). 4. Tsavachidou, D., et al., Cancer Res. 64(16):5556-5559 (2004). 5. Gear, H., et al., Invest. Ophthalmol. Vis. Sci. 45(8):2484-2488 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 33188459
• NCBI GeneID: 673
• NCBI Accession #: NP_004324
• NCBI GenBank Nucleotide #: NP_004324.2
• UniProt Accession #: P15056; Q13878; Q3MIN6; Q9UDP8; Q9Y6T3; A4D1T4; B6HY61; B6HY62; B6HY63; B6HY64; B6HY65; B6HY66
• Molecular Weight: 40.6 kDa (360aa)
• NCBI Official Full Name: serine/threonine-protein kinase B-raf isoform 1
• NCBI Official Synonym Full Names: B-Raf proto-oncogene, serine/threonine kinase
• NCBI Official Symbol: BRAF
• NCBI Official Synonym Symbols: NS7; B-raf; BRAF1; RAFB1; B-RAF1
• NCBI Protein Information: serine/threonine-protein kinase B-raf
• UniProt Protein Name: Serine/threonine-protein kinase B-raf
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: BRAF
• UniProt Synonym Gene Names: BRAF1; RAFB1
• UniProt Entry Name: BRAF_HUMAN

NCBI Description

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

Uniprot Description

BRAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib.

Protein type: Protein kinase, TKL; Kinase, protein; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; TKL group; RAF family

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: neuron projection; mitochondrion; plasma membrane; cytosol; nucleus

Molecular Function: small GTPase binding; identical protein binding; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; protein heterodimerization activity; calcium ion binding; ATP binding; protein kinase activity

Biological Process: response to peptide hormone stimulus; positive T cell selection; response to cAMP; fibroblast growth factor receptor signaling pathway; CD4-positive, alpha beta T cell differentiation; nerve growth factor receptor signaling pathway; activation of MAPKK activity; protein heterooligomerization; somatic stem cell maintenance; MAPKKK cascade; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; regulation of cell proliferation; synaptic transmission; organ morphogenesis; myeloid progenitor cell differentiation; small GTPase mediated signal transduction; positive regulation of stress fiber formation; visual learning; negative regulation of neuron apoptosis; negative regulation of apoptosis

Disease: Noonan Syndrome 1; Noonan Syndrome 7; Lung Cancer; Leopard Syndrome 3; Cardiofaciocutaneous Syndrome 1