Rabbit anti-Human MLK1 Polyclonal Antibody | anti-MAP3K9 antibody

MLK1 (Mitogen-activated protein kinase kinase kinase 9, Mixed lineage kinase 1)

Cat. Num. AAA646550

• Gene Names: MAP3K9; MLK1; MEKK9; PRKE1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: MLK1; Polyclonal Antibody; MLK1 (Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1); Anti -MLK1 (Mitogen-activated protein kinase kinase kinase 9; anti-MAP3K9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human MLK1. Species sequence homology: Rat, mouse, chimpanzee, rhesus macaque (100%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

• Applicable Applications for anti-MAP3K9 antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry: 1:300
• Immunogen: Synthetic linear peptide corresponding to human MLK1 (KLH).
• Positive Control: Colorectal adenocarcinoma tissue
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-MAP3K9 antibody

MLK1 (MAP3K9) is a serine-threonine kinase of the Mixed-Lineage Kinase (MLK) family capable of activating the c-Jun N-terminal Kinase (JNK)/Mitogen-Activated Protein Kinase (MAPK) pathway and regulating the other two prinicpal MAPK cascades, p38 and extracellular signal regulated kinase (ERK). Immunoblots and mass spectrometry show MLK is threonine phosphorylated in or near the activation loop. Studies show that MLKs play a role in the TGF-beta1-induced cell death mechanism.

Product Categories/Family for anti-MAP3K9 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 545746337
• NCBI GeneID: 4293
• NCBI Accession #: NP_001271159.1
• NCBI GenBank Nucleotide #: NM_001284230.1
• UniProt Accession #: P80192; Q0D2G7; Q6EH31; Q9H2N5; A3KN85
• Molecular Weight: 121,895 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 9 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 9
• NCBI Official Symbol: MAP3K9
• NCBI Official Synonym Symbols: MLK1; MEKK9; PRKE1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 9; mixed lineage kinase 1 (tyr and ser/thr specificity)
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 9
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MAP3K9
• UniProt Synonym Gene Names: MLK1; PRKE1
• UniProt Entry Name: M3K9_HUMAN

Uniprot Description

Function: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Ref.1 Ref.6 Ref.8

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Enzyme regulation: Homodimerization via the leucine zipper domains is required for autophosphorylation of multiple sites in the activation loop and subsequent activation. Autophosphorylation at Thr-312 is the key step in activation of MAP3K9/MLK1 and is required for full phosphorylation. Autophosphorylation at Thr-304 and Ser-308 have been shown to be of secondary importance in the activation of MAP3K9/MLK1. CEP-1347 and many indolocarbazole analogs have been shown to act as inhibitors of MAP3K9/MLK1 activity. Ref.1 Ref.5 Ref.7

Subunit structure: Homodimer

By similarity.

Tissue specificity: Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin. Ref.4

Post-translational modification: Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. Autophosphorylation also occurs on Thr-304 and Ser-308.

Involvement in disease: May play a role in esophageal cancer susceptibility and/or development.

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.Contains 1 protein kinase domain.Contains 1 SH3 domain.

Biophysicochemical propertiesKinetic parameters:KM=62 µM for ATP Ref.5KM=7 µM for myelin basic protein (MBP) as substrate

Sequence caution: The sequence AAI11408.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.