Western Blot (WB)
(Western blot analysis of ACER3 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). ACER3 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse ACER3 Polyclonal Antibody | anti-ACER3 antibody
ACER3, CT (Alkaline Dihydroceramidase SB89, Alkaline Phytoceramidase, aPHC, Alkaline Ceramidase 3, Alkaline CDase 3, AlkCDase 3, APHC, PHCA) (APC)
Gene Names
ACER3; APHC; PHCA
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
ACER3; Polyclonal Antibody; CT (Alkaline Dihydroceramidase SB89; Alkaline Phytoceramidase; aPHC; Alkaline Ceramidase 3; Alkaline CDase 3; AlkCDase 3; APHC; PHCA) (APC); anti-ACER3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ACER3. Species Crossreactivity: mouse.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-ACER3 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-1:500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human ACER3 (KLH).
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of ACER3 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). ACER3 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ACER3 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). ACER3 (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(ACER3 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ACER3 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-ACER3 antibody
References
1. Wheeler, H.E., et.al., PLoS Genet. 5(10), E1000685 (2009). 2. Mao, C. et.al., Biochim. Biophys. Acta 1781(9), 424-434 (2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,552 Da
NCBI Official Full Name
alkaline ceramidase 3
NCBI Official Synonym Full Names
alkaline ceramidase 3
NCBI Official Symbol
ACER3
NCBI Official Synonym Symbols
APHC; PHCA
NCBI Protein Information
alkaline ceramidase 3; alkCDase 3; alkaline CDase 3; alkaline phytoceramidase; phytoceramidase, alkaline; alkaline dihydroceramidase SB89
UniProt Protein Name
Alkaline ceramidase 3
Protein Family
UniProt Gene Name
ACER3
UniProt Synonym Gene Names
APHC; PHCA; AlkCDase 3; Alkaline CDase 3; aPHC
UniProt Entry Name
ACER3_HUMAN
Uniprot Description
ACER3: Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity. Belongs to the alkaline ceramidase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; EC 3.5.1.-; Hydrolase; Lipid Metabolism - sphingolipid
Chromosomal Location of Human Ortholog: 11q13.5
Cellular Component: endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; integral to Golgi membrane
Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; positive regulation of cell proliferation; sphingosine biosynthetic process; ceramide metabolic process
Research Articles on ACER3
Similar Products
Product Notes
The ACER3 acer3 (Catalog #AAA6463198) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACER3, CT (Alkaline Dihydroceramidase SB89, Alkaline Phytoceramidase, aPHC, Alkaline Ceramidase 3, Alkaline CDase 3, AlkCDase 3, APHC, PHCA) (APC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ACER3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-1:500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ACER3 acer3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACER3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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