Rabbit MAP1LC3A Polyclonal Antibody | anti-MAP1LC3A antibody

MAP1LC3A (Microtubule-associated Proteins 1A/1B Light Chain 3A, Autophagy-related Protein LC3 A, Autophagy-related Ubiquitin-like Modifier LC3 A, MAP1 Light Chain 3-like Protein 1, MAP1A/MAP1B Light Chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated Pro

Cat. Num. AAA646231

• Gene Names: MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• Reactivity: Bovine, Human, Mouse, Rat, Xenopus
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: MAP1LC3A; Polyclonal Antibody; MAP1LC3A (Microtubule-associated Proteins 1A/1B Light Chain 3A; Autophagy-related Protein LC3 A; Autophagy-related Ubiquitin-like Modifier LC3 A; MAP1 Light Chain 3-like Protein 1; MAP1A/MAP1B Light Chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated Pro; Anti -MAP1LC3A (Microtubule-associated Proteins 1A/1B Light Chain 3A; anti-MAP1LC3A antibody

• Host: Rabbit
• Reactivity: Bovine, Human, Mouse, Rat, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LC3. Species Crossreactivity: rat, mouse and bovine, Xenopus.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Triscitrate/ phosphate, pH 7-8, 0.1% sodium azide.

• Applicable Applications for anti-MAP1LC3A antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot.; Dilution: Immunohistochemistry: 1:200-1:400; Western Blot: 2ug/ml; Immunoprecipitation: 20ug/500ug of protein
• Immunogen: Synthetic peptide corresponding to an internal portion, aa50-125, from human LC3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-MAP1LC3A antibody

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

Product Categories/Family for anti-MAP1LC3A antibody

Antibodies; Abs to Microtubule-associated Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 31563518
• NCBI GeneID: 84557
• NCBI Accession #: NP_852610.1
• NCBI GenBank Nucleotide #: NM_181509.2
• UniProt Accession #: Q9H492; Q9BXW5; E1P5P4; E1P5P5
• Molecular Weight: 14,272 Da
• NCBI Official Full Name: microtubule-associated proteins 1A/1B light chain 3A isoform b
• NCBI Official Synonym Full Names: microtubule-associated protein 1 light chain 3 alpha
• NCBI Official Symbol: MAP1LC3A
• NCBI Official Synonym Symbols: LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3A; MAP1A/MAP1B LC3 A; MAP1A/1B light chain 3 A; MAP1A/MAP1B light chain 3 A; MAP1 light chain 3-like protein 1; autophagy-related ubiquitin-like modifier LC3 A
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3A
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP1LC3A
• UniProt Synonym Gene Names: MAP1A/MAP1B LC3 A
• UniProt Entry Name: MLP3A_HUMAN

NCBI Description

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]

Uniprot Description

Function: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Ref.12

Subunit structure: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins

By similarity. Interacts with SQSTM1

By similarity. Interacts with TP53INP1 and TP53INP2. Directly interacts with SQSTM1; this interaction leads to MAP1LC3A recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy. Interacts with ATG13 and ULK1. Ref.7 Ref.8 Ref.10 Ref.11 Ref.14 Ref.15

Subcellular location: Cytoplasm › cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle › autophagosome membrane; Lipid-anchor. Note: LC3-II binds to the autophagic membranes. Ref.1 Ref.10 Ref.11 Ref.13

Tissue specificity: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. Ref.1

Post-translational modification: The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Ref.9The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.

Sequence similarities: Belongs to the ATG8 family.