Testing Data
(Representative blot from a previous lot. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A1125-36A (1:1000). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Arrow indicates Akt3/PKBg (~60kD).)
Rabbit anti-Human Akt3 Polyclonal Antibody | anti-Akt3 antibody
Akt3 (RAC-PK Gamma) (Biotin)
WB: A 1:500-1:2000 dilution of this lot detected Akt3/PKBg in HEK293 cell lysates.
Applications are based on unconjugated antibody.
Testing Data
(Representative blot from a previous lot. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A1125-36A (1:1000). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Arrow indicates Akt3/PKBg (~60kD).)
Testing Data
(Representative blot from a previous lot. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A1125-36A (1:1000). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Arrow indicates Akt3/PKBg (~60kD).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
Akt3: an AGC kinase that plays a critical role in controlling the balance between survival and apoptosis. Stimulated by platelet-derived growth factor, insulin, and insulin-like growth factor 1. Alternatively spliced isoforms have been described.
Protein type: Protein kinase, AGC; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; AKT family
Chromosomal Location of Human Ortholog: 1q44
Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity
Biological Process: mitochondrial genome maintenance; signal transduction; protein amino acid phosphorylation
Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 2
Research Articles on Akt3
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Product Notes
The Akt3 akt3 (Catalog #AAA6462184) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Akt3 (RAC-PK Gamma) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Akt3 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). IP: 5ul immunoprecipitated active Akt3/PKBg from 15nM IGF-I stimulated HEK293 cell lysates. The kinase activity of immunoprecipitated Akt3/PKBg was determined using Akt/SGK Specific Substrate Peptide Active kinase was detected by comparing the incorporation of [32P] into Akt/SGK Substrate Peptide as catalyzed by immunoprecipitates from IGF-I stimulated (15nM) HEK293 cell lysates (1mg). WB: A 1:500-1:2000 dilution of this lot detected Akt3/PKBg in HEK293 cell lysates. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Akt3 akt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Akt3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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