Rabbit anti-Human AKT3 Polyclonal Antibody | anti-AKT3 antibody

AKT3 (S472) (RAC-gamma Serine/Threonine-protein Kinase, RAC-PK-gamma, Protein Kinase Akt-3, Protein Kinase B, gamma, PKB gamma, STK-2, PKBG) (AP)

Cat. Num. AAA6462171

• Gene Names: AKT3; MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: AKT3; Polyclonal Antibody; AKT3 (S472) (RAC-gamma Serine/Threonine-protein Kinase; RAC-PK-gamma; Protein Kinase Akt-3; Protein Kinase B; gamma; PKB gamma; STK-2; PKBG) (AP); anti-AKT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AKT3.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-AKT3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding S472 of human AKT3 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of AKT3 (arrow) using rabbit polyclonal AKT3 Antibody (S472). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT3 gene (Lane 2) (Origene Technologies).)

Product Categories/Family for anti-AKT3 antibody

Antibodies; Protein Kinases

References

1. Xu, Z., et al., Biochem. Biophys. Res. Commun. 312(2):388-396 (2003). 2. Tiwari, G., et al., Mol. Cancer Res. 1(6):475-484 (2003). 3. Brozinick, J.T. Jr., et al., Diabetes 52(4):935-941 (2003). 4. Deregibus, M.C., et al., J. Biol. Chem. 277(28):25195-25202 (2002). 5. Brodbeck, D., et al., J. Biol. Chem. 276(31):29550-29558 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 4885549
• NCBI GeneID: 10000
• NCBI Accession #: NP_005456
• NCBI GenBank Nucleotide #: NM_005465.4
• UniProt Accession #: Q9Y243; Q0VAA6; Q5VTI1; Q5VTI2; Q96QV3; Q9UFP5
• Molecular Weight: ~84.0 kDa
• NCBI Official Full Name: RAC-gamma serine/threonine-protein kinase isoform 1
• NCBI Official Synonym Full Names: v-akt murine thymoma viral oncogene homolog 3
• NCBI Official Symbol: AKT3
• NCBI Official Synonym Symbols: MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
• NCBI Protein Information: RAC-gamma serine/threonine-protein kinase
• UniProt Protein Name: RAC-gamma serine/threonine-protein kinase
• Protein Family: RAC-gamma serine/threonine-protein kinase
• UniProt Gene Name: AKT3
• UniProt Synonym Gene Names: PKBG; PKB gamma
• UniProt Entry Name: AKT3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Akt3: an AGC kinase that plays a critical role in controlling the balance between survival and apoptosis. Stimulated by platelet-derived growth factor, insulin, and insulin-like growth factor 1. Alternatively spliced isoforms have been described.

Protein type: Protein kinase, AGC; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; AKT family

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: mitochondrial genome maintenance; signal transduction; protein amino acid phosphorylation

Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 2