Rabbit anti-Human AKAP3 Polyclonal Antibody | anti-AKAP3 antibody

AKAP3, ID (A-kinase Anchor Protein 3, AKAP-3, Protein Kinase A-anchoring Protein 3, PRKA3, A-kinase Anchor Protein 110kD, AKAP 110, Sperm Oocyte-binding Protein, Fibrousheathin-1, Fibrousheathin I, Fibrous Sheath Protein Of 95kD, FSP95, Cancer/Testis Anti

Cat. Num. AAA6461955

• Gene Names: AKAP3; CT82; SOB1; FSP95; PRKA3; AKAP110; AKAP 110
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: AKAP3; Polyclonal Antibody; ID (A-kinase Anchor Protein 3; AKAP-3; Protein Kinase A-anchoring Protein 3; PRKA3; A-kinase Anchor Protein 110kD; AKAP 110; Sperm Oocyte-binding Protein; Fibrousheathin-1; Fibrousheathin I; Fibrous Sheath Protein Of 95kD; FSP95; Cancer/Testis Anti; anti-AKAP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AKAP3.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-AKAP3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human AKAP3 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of AKAP3 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). AKAP3 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-AKAP3 antibody

Antibodies; Protein Kinases

References

1. Lefevre, A., et.al., Biochem. Biophys. Res. Commun. 259(1), 60-66 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 21493041
• NCBI GeneID: 10566
• NCBI Accession #: NP_006413.2
• NCBI GenBank Nucleotide #: NM_006422.2
• UniProt Accession #: O75969; O75945; Q86X01; Q9UM61
• Molecular Weight: 94,751 Da
• NCBI Official Full Name: A-kinase anchor protein 3
• NCBI Official Synonym Full Names: A kinase (PRKA) anchor protein 3
• NCBI Official Symbol: AKAP3
• NCBI Official Synonym Symbols: CT82; SOB1; FSP95; PRKA3; AKAP110; AKAP 110
• NCBI Protein Information: A-kinase anchor protein 3; fibrousheathin I; fibrousheathin-1; cancer/testis antigen 82; sperm oocyte-binding protein 1; A-kinase anchor protein, 110kDa; Fibrous Sheath Protein of 95 kDa; protein kinase A-anchoring protein 3; protein kinase A binding prot
• UniProt Protein Name: A-kinase anchor protein 3
• Protein Family: A-kinase anchor protein
• UniProt Gene Name: AKAP3
• UniProt Synonym Gene Names: AKAP110; SOB1; AKAP-3; AKAP 110; CT82; FSP95; PRKA3
• UniProt Entry Name: AKAP3_HUMAN

NCBI Description

This gene encodes a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. The encoded protein is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. This protein is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction. [provided by RefSeq, May 2013]

Uniprot Description

AKAP3: May function as a regulator of both motility- and head- associated functions such as capacitation and the acrosome reaction. Belongs to the AKAP110 family.

Protein type: Adaptor/scaffold; Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: cytoplasm; acrosome; nucleus

Molecular Function: protein binding; protein kinase A binding

Biological Process: protein localization; single fertilization; acrosome reaction; transmembrane receptor protein serine/threonine kinase signaling pathway; cell motility