Rabbit anti-Human ACHY Polyclonal Antibody | anti-ACHY antibody

ACHY, NT (Adenosylhomocysteinase, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, SAHH) (MaxLight 750)

Cat. Num. AAA6461916

• Gene Names: AHCY; SAHH; adoHcyase
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ACHY; Polyclonal Antibody; NT (Adenosylhomocysteinase; AdoHcyase; S-adenosyl-L-homocysteine hydrolase; SAHH) (MaxLight 750); anti-ACHY antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AHCY.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-ACHY antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human AHCY (KLH).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-AHCY Pab in 293 cell line lysates (35ug/lane).AHCY (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AKR1A1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-ACHY antibody

Antibodies; Enzymes

References

1. Yideng, J., DNA Cell Biol. 26(8), 603-611 (2007). 2. Arredondo-Vega, F.X., Ann. Hum. Genet. 53(PT 2), 157-167 (1989). 3. Li, Q.S., Biochemistry 47(17), 4983-4991 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 9951915
• NCBI GeneID: 191
• NCBI Accession #: NP_000678
• NCBI GenBank Nucleotide #: NM_000687.3
• UniProt Accession #: P23526; Q96A36; A8K307; B3KUN3; E1P5P2; F5H737
• Molecular Weight: 49.8 kDa (452aa) confirmed by MALDI-TOF
• NCBI Official Full Name: adenosylhomocysteinase isoform 1
• NCBI Official Synonym Full Names: adenosylhomocysteinase
• NCBI Official Symbol: AHCY
• NCBI Official Synonym Symbols: SAHH; adoHcyase
• NCBI Protein Information: adenosylhomocysteinase
• UniProt Protein Name: Adenosylhomocysteinase
• UniProt Gene Name: AHCY
• UniProt Synonym Gene Names: SAHH; AdoHcyase
• UniProt Entry Name: SAHH_HUMAN

NCBI Description

S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

SAHH: Adenosylhomocysteine is a competitive inhibitor of S- adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD). A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy. Belongs to the adenosylhomocysteinase family.

Protein type: Other Amino Acids Metabolism - selenoamino acid; EC 3.3.1.1; Hydrolase; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: neuron projection; cytoplasm; melanosome; nucleus; cytosol

Molecular Function: identical protein binding; adenyl nucleotide binding; adenosylhomocysteinase activity

Biological Process: methylation; circadian sleep/wake cycle; sulfur amino acid metabolic process; S-adenosylmethionine cycle; xenobiotic metabolic process; response to hypoxia; S-adenosylhomocysteine catabolic process; one-carbon compound metabolic process; chronic inflammatory response to antigenic stimulus; response to nutrient

Disease: Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency