Western Blot (WB)
(Western Blot analysis in HepG2 cell line lysates (35ug/lane) using MBS627866 (8ug/ml). AGXT (arrow) was detected using the purified Pab.)
Rabbit anti-Human AGXT Polyclonal Antibody | anti-AGXT antibody
AGXT, ID (Serine-Pyruvate Aminotransferase, SPT, Alanine-Glyoxylate Aminotransferase, AGT, SPAT, AGT1) (AP)
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western Blot analysis in HepG2 cell line lysates (35ug/lane) using MBS627866 (8ug/ml). AGXT (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western Blot analysis in HepG2 cell line lysates (35ug/lane) using MBS627866 (8ug/ml). AGXT (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western Blot analysis in human liver lysates (20ug/lane) using MBS627866 (1:8000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000. Predicted band size: 43kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot analysis in human liver lysates (20ug/lane) using MBS627866 (1:8000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000. Predicted band size: 43kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Immunohistochemistry in paraformaldehyde-fixed, paraffin-embedded sections of human hepatic carcinoma tissue sections usingMBS627866. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry in paraformaldehyde-fixed, paraffin-embedded sections of human hepatic carcinoma tissue sections usingMBS627866. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma using MBS627866 followed by peroxidase conjugation of the secondary antibody and DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma using MBS627866 followed by peroxidase conjugation of the secondary antibody and DAB staining.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HepG2 cells (right histogram) using MBS627866 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HepG2 cells (right histogram) using MBS627866 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008]
Research Articles on AGXT
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Product Notes
The AGXT agxt (Catalog #AAA6461885) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AGXT, ID (Serine-Pyruvate Aminotransferase, SPT, Alanine-Glyoxylate Aminotransferase, AGT, SPAT, AGT1) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AGXT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-1:500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AGXT agxt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AGXT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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