Western Blot (WB)
(Western blot analysis of HIST1H1B Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane).HIST1H1B (arrow) was detected using the purified Pab.)
Rabbit anti-Human HIST1H1B Polyclonal Antibody | anti-HIST1H1B antibody
HIST1H1B, NT (HIST1H1B, H1F5, Histone H1.5, Histone H1a)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Western Blot (WB)
(Western blot analysis of HIST1H1B Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane).HIST1H1B (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of HIST1H1B Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane).HIST1H1B (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(HIST1H1B Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(HIST1H1B Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation
By similarity.
Subcellular location: Nucleus. Chromosome. Note: According to Ref.9 more commonly found in heterochromatin. According to Ref.7 associates with actively transcribed chromatin and not heterochromatin. Ref.7 Ref.8 Ref.9
Tissue specificity: Ubiquitous. Expressed in the majority of the cell lines tested and in testis. Ref.1
Domain: The C-terminal domain is required for high-affinity binding to chromatin
By similarity.
Post-translational modification: H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter
By similarity. Phosphorylated at Thr-11 by GSK3B during mitosis in prometaphase and dephosphorylated in telophase. Ref.13
Sequence similarities: Belongs to the histone H1/H5 family.Contains 1 H15 (linker histone H1/H5 globular) domain.
Research Articles on HIST1H1B
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Product Notes
The HIST1H1B hist1h1b (Catalog #AAA645866) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIST1H1B, NT (HIST1H1B, H1F5, Histone H1.5, Histone H1a) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HIST1H1B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the HIST1H1B hist1h1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIST1H1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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