Rabbit Smac Polyclonal Antibody | anti-DIABLO antibody

Smac, CT (DIABLO, Direct IAP Binding Protein of Low Pi, Second Mitochondrial Activator of Cell Death)

Cat. Num. AAA645561

• Gene Names: DIABLO; SMAC; DFNA64
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Neutralization Assay
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Smac; Polyclonal Antibody; CT (DIABLO; Direct IAP Binding Protein of Low Pi; Second Mitochondrial Activator of Cell Death); Anti -Smac; anti-DIABLO antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes a 25kD band. Species Crossreactivity: Human and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

• Applicable Applications for anti-DIABLO antibody: ELISA (EL/EIA), Western Blot (WB), Neutralization
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 0.5-1ug/ml; Optimal dilutions to be determined by the researcher.
• Immunogen: Raised against a peptide (SDEGADQEEEAYLRED) corresponding to amino acids 222 to 237 of murine Smac/DIABLO (2).
• Positive Control: Mouse heart tissue lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of Smac in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide (2411P) and in rat heart tissue lysate with MBS645561 at 1ug/ml)

Immunohistochemistry (IHC)

(Immunohistochemical staining of mouse spleen tissue using MBS645561 at 2ug/ml)

Related Product Information for anti-DIABLO antibody

The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identi ed and designated Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3-6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2).

Product Categories/Family for anti-DIABLO antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 9454219
• NCBI GeneID: 56616
• UniProt Accession #: Q9NR28; Q6W3F3; Q96LV0; Q9BT11; Q9HAV6; B2RDQ0
• Molecular Weight: 27,131 Da
• NCBI Official Full Name: Smac
• NCBI Official Synonym Full Names: diablo, IAP-binding mitochondrial protein
• NCBI Official Symbol: DIABLO
• NCBI Official Synonym Symbols: SMAC; DFNA64
• NCBI Protein Information: diablo homolog, mitochondrial; direct IAP-binding protein with low pI; second mitochondria-derived activator of caspase
• UniProt Protein Name: Diablo homolog, mitochondrial
• Protein Family: Diablo
• UniProt Gene Name: DIABLO
• UniProt Synonym Gene Names: SMAC; Smac
• UniProt Entry Name: DBLOH_HUMAN

NCBI Description

This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]

Uniprot Description

Function: Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. Ref.1 Ref.3 Ref.7

Subunit structure: Homodimer. Interacts with NGFRAP1/BEX3

By similarity. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin. Ref.7 Ref.8 Ref.10

Subcellular location: Mitochondrion. Note: Released into the cytosol when cells undergo apoptosis. Ref.3

Tissue specificity: Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. Ref.1 Ref.3

Domain: The mature N-terminus mediates interaction with XIAP/BIRC4.

Post-translational modification: Ubiquitinated by BIRC7/livin. Ref.8

Involvement in disease: Deafness, autosomal dominant, 64 (DFNA64) [MIM:614152]: A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.13