Rabbit anti-Human CHMP4A Polyclonal Antibody | anti-CHMP4A antibody

CHMP4A (Charged Multivesicular body Protein 4a, Chromatin-modifying Protein 4a, CHMP4a, SNF7 Homolog Associated with Alix-2, SNF7-1, hSnf-1, Vacuolar Protein Sorting-Associated Protein 32-1, Vps32-1, hVps32-1, CHMP4A, C14orf123, SHAX2) (APC)

Cat. Num. AAA6455085

• Gene Names: CHMP4A; SNF7; CHMP4; SHAX2; CHMP4B; SNF7-1; VPS32A; HSPC134; VPS32-1; C14orf123
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CHMP4A; Polyclonal Antibody; CHMP4A (Charged Multivesicular body Protein 4a; Chromatin-modifying Protein 4a; CHMP4a; SNF7 Homolog Associated with Alix-2; SNF7-1; hSnf-1; Vacuolar Protein Sorting-Associated Protein 32-1; Vps32-1; hVps32-1; C14orf123; SHAX2) (APC); anti-CHMP4A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CHMP4A. Species Crossreactivity: human
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CHMP4A antibody: Western Blot (WB)
• Application Notes: WB: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: KLH conjugated synthetic peptide corresponding to aa205-239 at the C-terminal region of human CHMP4A.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Categories/Family for anti-CHMP4A antibody

Antibodies; Transport Proteins

References

Katoh K., et al. J. Biol. Chem. 278:39104-39113(2003). Peck J.W., et al. Biochem. J. 377:693-700(2004). Li Y., et al. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases. Zhang Q.-H., et al. Genome Res. 10:1546-1560(2000). Li W.B., et al. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 40548422
• NCBI GeneID: 29082
• NCBI Accession #: NP_054888.2
• NCBI GenBank Nucleotide #: NM_014169.3
• UniProt Accession #: Q9BY43; Q14D22; Q32Q79; Q86SZ8; Q96QJ9; Q9P026
• Molecular Weight: 29,804 Da
• NCBI Official Full Name: charged multivesicular body protein 4a
• NCBI Official Synonym Full Names: charged multivesicular body protein 4A
• NCBI Official Symbol: CHMP4A
• NCBI Official Synonym Symbols: SNF7; CHMP4; SHAX2; CHMP4B; SNF7-1; VPS32A; HSPC134; VPS32-1; C14orf123
• NCBI Protein Information: charged multivesicular body protein 4a; SNF7 homolog associated with Alix-2; Snf7 homologue associated with Alix 2; chromatin modifying protein 4A; vacuolar protein sorting-associated protein 32-1
• UniProt Protein Name: Charged multivesicular body protein 4a
• Protein Family: Charged multivesicular body protein
• UniProt Gene Name: CHMP4A
• UniProt Synonym Gene Names: C14orf123; SHAX2; CHMP4a; hSnf-1; Vps32-1; hVps32-1
• UniProt Entry Name: CHM4A_HUMAN

NCBI Description

CHMP4A belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008]

Uniprot Description

CHMP4A: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4A filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release. Belongs to the SNF7 family.

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: internal side of plasma membrane; cytoplasmic vesicle membrane; membrane coat; late endosome membrane; cytoplasm; plasma membrane; midbody; nucleus; cytosol; endosome

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; lipid binding; ATPase binding

Biological Process: protein polymerization; cell separation during cytokinesis; viral reproduction; vacuolar transport; membrane invagination; viral infectious cycle; posttranslational protein targeting to membrane; endosome transport; mitotic metaphase plate congression; nuclear organization and biogenesis; protein homooligomerization; membrane budding