Rabbit anti-Human CASP8 Polyclonal Antibody | anti-CASP8 antibody

CASP8, CT (Caspase-8, CASP-8, ICE-like Apoptotic Protease 5, MORT1-associated CED-3 Homolog, MACH, FADD-homologous ICE/CED-3-like Protease, FADD-like ICE, FLICE, Apoptotic Cysteine Protease, Apoptotic Protease Mch-5, CAP4, Caspase-8 Subunit p18, Caspase-8

Cat. Num. AAA644805

• Gene Names: CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: CASP8; Polyclonal Antibody; CT (Caspase-8; CASP-8; ICE-like Apoptotic Protease 5; MORT1-associated CED-3 Homolog; MACH; FADD-homologous ICE/CED-3-like Protease; FADD-like ICE; FLICE; Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; CAP4; Caspase-8 Subunit p18; Caspase-8; Anti -CASP8; anti-CASP8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CASP8.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-CASP8 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunofluorescence, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100; Immunohistochemistry: 1:10-1:50; Immunofluorescence: 1:10-1:50
• Immunogen: Synthetic peptide selected from the C-terminal region of human CASP8 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CASP8 antibody (C-term) in Hela cell line lysates (35ug/lane). CASP8 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CASP8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of hela cells using CASP8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Immunofluorescence analysis of CASP8 Antibody (C-term) with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CASP8 Antibody (C-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-CASP8 antibody

CASP8 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases.

Product Categories/Family for anti-CASP8 antibody

Antibodies; Abs to Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 122056476
• NCBI GeneID: 841
• NCBI Official Full Name: caspase-8 isoform G
• NCBI Official Synonym Full Names: caspase 8, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP8
• NCBI Official Synonym Symbols: CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
• NCBI Protein Information: caspase-8
• Protein Family: Caspase