Rabbit anti-Rat Spinophilin Polyclonal Antibody | anti-PPP1R9B antibody

Spinophilin (Neurabin-2, Neurabin-II, Protein Phosphatase 1 Regulatory Subunit 9B, PPP1R9B, PPP1R6) (Biotin)

Cat. Num. AAA6444811

• Gene Names: PPP1R9B; Spn; SPINO; PPP1R6; PPP1R9
• Reactivity: Rat
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Spinophilin; Polyclonal Antibody; Spinophilin (Neurabin-2; Neurabin-II; Protein Phosphatase 1 Regulatory Subunit 9B; PPP1R9B; PPP1R6) (Biotin); anti-PPP1R9B antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Spinophilin.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PPP1R9B antibody: Western Blot (WB)
• Application Notes: WB: 0.05-0.1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to rat Spinophilin.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Rabbit anti-Spinophilin Western blot of rat brain lysate.)

Product Categories/Family for anti-PPP1R9B antibody

Antibodies; Enzymes; Phosphatase

References

1. Increased expression of Kalirin-9 in the auditory cortex of schizophrenia subjects: its role in dendritic pathology. Anthony J Deo, Michael E Cahill, Siyu Li, Isaac Goldszer, Ruth Henteleff, Jon-Eric Vanleeuwen, Igor Rafalovich, Ruoqi Gao, Erin K Stachowski, Allan R Sampson, David A Lewis, Peter Penzes, Robert A Sweet (2012) Neurobiology of disease.45. 2. Down-regulation of spinophilin in lung tumours contributes to tumourigenesis. Sonia Molina-Pinelo, Irene Ferrer, Carmen Blanco-Aparicio, Sandra Peregrino, Maria Dolores Pastor, Juan Alvarez-Vega, Rocio Suarez, Mar Verge, Juan J Marin, Javier Hernandez-Losa, Santiago Ramon y Cajal, Luis Paz-Ares, Amancio Carnero (2011) The Journal of pathology.225. 3. Spinophilin acts as a tumor suppressor by regulating Rb phosphorylation. Irene Ferrer, Carmen Blanco-Aparicio, Sandra Peregrina, Marta Cañamero, Jesús Fominaya, Yolanda Cecilia, Matilde Lleonart, Javier Hernandez-Losa, Santiago Ramon y Cajal, Amancio Carnero (2011) Cell cycle (Georgetown, Tex.).10. 4. Spinophilin loss contributes to tumorigenesis in vivo. Irene Ferrer, Sandra Peregrino, Marta Cañamero, Yolanda Cecilia, Carmen Blanco-Aparicio, Amancio Carnero (2011) Cell cycle (Georgetown, Tex.).10. 5. A sensitizing D-amphetamine dose regimen induces long-lasting spinophilin and VGLUT1 protein upregulation in the rat diencephalon. Steven R Boikess, Steven J O'Dell, John F Marshall (2010). Neuroscience letters.469. 6. Reduced dendritic spine density in auditory cortex of subjects with schizophrenia. Robert A Sweet, Ruth A Henteleff, Wei Zhang, Allan R Sampson, David A Lewis (2009). Neuropsychopharmacology: official publication of the American College of Neuropsychopharmacology.34.

NCBI and Uniprot Product Information

• NCBI GI #: 90101416
• NCBI GeneID: 84687
• UniProt Accession #: Q96SB3; Q8TCR9
• Molecular Weight: 815
• NCBI Official Full Name: Neurabin-2
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory subunit 9B
• NCBI Official Symbol: PPP1R9B
• NCBI Official Synonym Symbols: Spn; SPINO; PPP1R6; PPP1R9
• NCBI Protein Information: neurabin-2; neurabin-II; protein phosphatase 1, regulatory (inhibitor) subunit 9B; protein phosphatase 1, regulatory subunit 9B, spinophilin
• UniProt Protein Name: Neurabin-2
• Protein Family: Neurabin
• UniProt Gene Name: PPP1R9B
• UniProt Synonym Gene Names: PPP1R6
• UniProt Entry Name: NEB2_HUMAN

NCBI Description

This gene encodes a scaffold protein that functions as a regulatory subunit of protein phosphatase 1a. Expression of this gene is particularly high in dendritic spines, suggesting that the encoded protein may play a role in receiving signals from the central nervous system. The encoded protein has putative tumor suppressor function and decreased expression has been observed in tumors. [provided by RefSeq, Feb 2014]

Uniprot Description

Spinophilin: actin-binding regulatory subunit of protein phosphatase 1 (PP1). The actin-binding domain of spinophilin is necessary and sufficient for targeting of spinophilin to dendrites and dendritic spines. May play a role in establishing a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Interacts with p14-ARF. Contains one PDZ/DHR domain.

Protein type: Protein phosphatase, regulatory subunit; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: nucleoplasm; cortical actin cytoskeleton; adherens junction; lamellipodium; cytoplasm; dendritic spine; plasma membrane; synapse; protein phosphatase type 1 complex; filopodium

Molecular Function: protein binding; protein phosphatase inhibitor activity; actin binding; protein phosphatase 1 binding

Biological Process: regulation of synaptic transmission; regulation of protein amino acid phosphorylation; cell migration; regulation of cell growth by extracellular stimulus; filopodium formation; RNA splicing; regulation of exit from mitosis; calcium-mediated signaling; actin filament organization; regulation of cell proliferation; negative regulation of catalytic activity; dendrite development; negative regulation of cell growth; cell cycle arrest