Western Blot (WB)
(Western Blot analysis of DPH5 expression in transfected 293T cell line by DPH5 polyclonal antibody. Lane 1: DPH5 transfected lysate (31.35kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human DPH5 Polyclonal Antibody | anti-DPH5 antibody
DPH5 (Diphthine Synthase, Diphthamide Biosynthesis Methyltransferase, AD-018, CGI-30, HSPC143, NPD015)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot analysis of DPH5 expression in transfected 293T cell line by DPH5 polyclonal antibody. Lane 1: DPH5 transfected lysate (31.35kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of DPH5 expression in transfected 293T cell line by DPH5 polyclonal antibody. Lane 1: DPH5 transfected lysate (31.35kD). Lane 2: Non-transfected lysate.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
DPH5: S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF- 2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis. Belongs to the diphthine synthase family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Methyltransferase; EC 2.1.1.98
Chromosomal Location of Human Ortholog: 1p21.2
Cellular Component: cytosol
Molecular Function: diphthine synthase activity
Biological Process: methylation; cellular protein metabolic process; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; post-translational protein modification
Research Articles on DPH5
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Product Notes
The DPH5 dph5 (Catalog #AAA644190) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DPH5 (Diphthine Synthase, Diphthamide Biosynthesis Methyltransferase, AD-018, CGI-30, HSPC143, NPD015) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DPH5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the DPH5 dph5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MLYLIGLGLG DAKDITVKGL EVVRRCSRVY LEAYTSVLTV GKEALEEFYG RKLVVADREE VEQEADNILK DADISDVAFL VVGDPFGATT HSDLVLRATK LGIPYRVIHN ASIMNAVGCC GLQLYKFGET VSIVFWTDTW RPESFFDKVK KNRQNGMHTL CLLDIKVKEQ SLENLIKGRK IYEPPRYMSV NQAAQQLLEI VQNQRIRGEE PAVTEETLCV GLARVGADDQ KIAAGTLRQM CTVDLGEPLH SLIITGGSIH PMEMEMLSLF SIPENSSESQ SINGL. It is sometimes possible for the material contained within the vial of "DPH5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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