Rabbit anti-Human TDG Polyclonal Antibody | anti-TDG antibody

TDG (Teratocarcinoma Derived Growth Factor, hTDG) (HRP)

Cat. Num. AAA6440217

• Gene Names: TDG; hTDG
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: TDG; Polyclonal Antibody; TDG (Teratocarcinoma Derived Growth Factor; hTDG) (HRP); anti-TDG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TDG.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-TDG antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human TDG.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TDG antibody.)

Product Categories/Family for anti-TDG antibody

Antibodies; Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 59853162
• NCBI GeneID: 6996
• NCBI Accession #: NP_003202.3
• NCBI GenBank Nucleotide #: NM_003211.4
• UniProt Accession #: Q13569; Q8IUZ6; Q8IZM3
• Molecular Weight: 46,053 Da
• NCBI Official Full Name: G/T mismatch-specific thymine DNA glycosylase
• NCBI Official Synonym Full Names: thymine-DNA glycosylase
• NCBI Official Symbol: TDG
• NCBI Official Synonym Symbols: hTDG
• NCBI Protein Information: G/T mismatch-specific thymine DNA glycosylase
• UniProt Protein Name: G/T mismatch-specific thymine DNA glycosylase
• Protein Family: G/T mismatch-specific thymine DNA glycosylase
• UniProt Gene Name: TDG
• UniProt Synonym Gene Names: hTDG
• UniProt Entry Name: TDG_HUMAN

NCBI Description

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008]

Uniprot Description

TDG: In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar- phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. Belongs to the TDG/mug DNA glycosylase family.

Protein type: EC 3.2.2.29; Hydrolase; DNA repair, damage; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: nucleoplasm; PML body; plasma membrane; nucleus

Molecular Function: protein domain specific binding; protein binding; protein homodimerization activity; structure-specific DNA binding; protein kinase C binding; SUMO binding; DNA N-glycosylase activity; double-stranded DNA binding; damaged DNA binding; transcription factor binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity; mismatched DNA binding

Biological Process: transcription from RNA polymerase II promoter; embryonic development; mismatch repair; base-excision repair, AP site formation; base-excision repair; depyrimidination; gene expression; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; negative regulation of protein binding; DNA repair; regulation of gene expression, epigenetic